Sekvenciranje RNA in primer uporabe v diagnostiki
RNA sequencing and example of diagnostic use

slovenian

2024

differential gene expression analysis   RNA sequencing

Narodna in univerzitetna knjižnica
(Obvezni izvod spletne publikacije)

RNA sequencing is an advanced molecular technique that allows the transcriptomes to be studied in detail, representing a remarkable advance in transcriptome studies. The first step in RNA sequencing is the isolation of RNA from a biological sample, followed by RNA library preparation, sequencing, and bioinformatics analysis. The main objective is to quantify and compare gene expression between different conditions, providing insights into the biological function of the genes analyzed. Despite its numerous advantages, RNA sequencing also has certain limitations and drawbacks. These include the instability of RNA and its susceptibility to degradation by RNAses, which are difficult to remove and require careful sample preparation. The clinical application of this technology is demonstrated using the example of a patient with B-cell acute lymphoblastic leukaemia.

17.01.2025