Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings.

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URN

URN:NBN:SI:DOC-G2LEQPEJ

COBISSID

3081864

Added

15.01.2013

Metadata

Citation

APA:

Dušanić, Daliborka, Bolha, Luka, Narat, Mojca, Oven, Irena (2012). Setting up a gene expression study for tissue cells by method of quantitative real-time PCR. Acta agriculturae Slovenica, volume 100, issue 1, str. 19-28. URN:NBN:SI:DOC-G2LEQPEJ from http://www.dlib.si

MLA:

Dušanić, Daliborka, Bolha, Luka, Narat, Mojca, Oven, Irena. "Setting up a gene expression study for tissue cells by method of quantitative real-time PCR." Acta agriculturae Slovenica volume 100. issue 1 (2012) str. 19-28.
<http://www.dlib.si/?URN=URN:NBN:SI:DOC-G2LEQPEJ>

Acta agriculturae Slovenica
2004
(Assembly record)

številka

Acta agriculturae Slovenica
2012
(Complete issue)