Eritropoetin, epoetini in njihova detekcija
Erythropoietin, epoetins and detection

slovenian

2009

volume 60, issue 5

Slovensko farmacevtsko društvo

Erythropoietin (EPO) is an endogenous hormone synthesized in special epithelial cells in renal peritubular capillaries. It is the main factor in human and animal erythropoiesis. EPO is synthesised in kidney (90 %) and some in liver and other organs like brain (10 %). Recombinant human erythropoietin (rHuEPO) used in medicine, was introduced to the market in early nineties. rHuEPO improved quality of life of patients with kidney failure and anaemic patients, which we re receiving blood transfusion. However, because of its positive influence some athletes from different sports abu se it. EPO acts as a stimulus for production of red blood celis, leading to more efficient oxygendelivery to museles. Therefare the International Olympic Comity banned it in 1987. Detection method that could differentiate between endogenous and exogenou s EPO was developed in 2000, when the method called isoelectric focusing (IEF) was first used. The problem of detection of rHuEPO is its cornplexity and structural similarity to endogenous EPO and al so the low concentration of EPO in human blood and urine.

21.07.2011