413 Documenta Praehistorica XLIII (2016) Comparing different sampling methods in order to reconstruct plant economies at the Eneolithic lake dwelling site Stare gmajne, Slovenia Tja[a Tolar, Anton Velu[;ek ZRC SAZU, Institute of Archaeology, Ljubljana, SI tjasa.tolar@zrc-sazu.si Introduction The Stare gmajne site is an Eneolithic (Copper Age) pile dwelling site in Slovenia dated to the 4th mil- lennium BC. According to dendrochronological and radiocarbon dating, it was settled many times (∞u- far et al. 2015). The earliest settlement ended be- fore 3300 cal BC, and the latest around 3110 cal BC (∞ufar et al. 2009; 2010; 2015). The site became more widely known when a wooden wheel with an axle, the oldest found to date, was discovered in 2002. Taking into account its radiocarbon dating and the age of the settlement where it was found, it is c. 5150 years old (Velu∏≠ek et al. 2009). In 2006 and 2007, the site was excavated; two 15m2 trenches (Velu∏≠ek 2009.54–55) were opened and researched in detail (see Velu∏≠ek 2009). During the excavation in 2006, a large loom-weight was found in the latest settlement phase, which is dendrochronologically dated to between 3160 and 3100±14 cal BC. The bottom of the loom-weight was accidentally broken or fell off during the exca- vation. It was carefully stored as a judgement sam- ple for analysis in the lab. Waterlogged clayey mate- rial was obtained from the interior of the broken loom-weight and analysed in detail (Tolar et al. 2016). In 2007, for the first time in Slovenia, excavations included appropriate sampling and treatment me- thods for archaeobotanical investigations of water- logged material (Tolar et al. 2010). Three 1m2 qua- ABSTRACT – The results of plant macroremains studies of the Eneolithic (c. 3160–3100 cal BC) lake- shore settlement at Stare gmajne on the Ljubljansko barje in Slovenia are presented. Archaeobotani- cal material was collected in two different ways: (1) systematic surface sampling from the cultural layer, and (2) judgement sampling from an incompletely burnt large loom-weight. The preservation state and the spectra of plant macroremains were different in both types of samples. The first study primarily deals with the waterlogged plant remains of various types and taxa, while the second deals with carbonised and half-carbonised cereal macroremains, mostly chaff. Both studies confirm the cultivation of main crops: emmer, einkorn and barley. IZVLE∞EK – Predstavljeni so rezultati analiz rastlinskih makroostankov z eneolitskega (ok. 3160– 3100 cal BC) koli∏≠a Stare gmajne z Ljubljanskega barja, Slovenija. Material za arheobotani≠ne raz- iskave smo pridobili po dveh metodah: (1) sistemati≠no povr∏insko vzor≠enje iz kulturne plasti in (2) vzor≠enje po presoji iz nepopolno ∫gane velike glinene ute∫i. Ohranjenost in spekter rastlinskih makroostankov iz obeh vzorcev se bistveno razlikujeta. Prva ∏tudija se ukvarja predvsem z ne-zogle- nelimi in z vodo prepojenimi makroostanki razli≠nih tipov in taksonov, medtem ko druga prou≠u- je v glavnem zoglenele in pol-zoglenele ostanke ∫it, predvsem plev. Obe ∏tudiji potrjujeta gojenje glav- nih kulturnih rastlin: dvozrnice, enozrnice in je≠mena. KEY WORDS – Eneolithic; pile dwelling; Slovenia; plant economy; taphonomy DOI> 10.4312\dp.43.20 Tja[a Tolar, Anton Velu[;ek 414 drants from the 15m2 trench with an approx. 46cm thick cultural layer were systematically surface sam- pled and archaeobotanicaly analysed (Fig. 1). Both the latest (upper 21cm of the sediment in the cultur- al layer) and the earliest (lower 25cm of the sedi- ment) settlement phases were investigated (Tolar et al. 2011). The present paper compares these two studies: sur- face sampled sediment samples from the latest cul- tural layer of the excavated trench in 2007 vs. judge- ment sampled material from the loom-weight. Both samples were waterlogged and therefore had great potential for archaeobotanical investigation. There are also important differences between the two. Although preserved in waterlogged conditions, the first study deals mainly with waterlogged plant macroremains that were deposited on the surface during the life of the settlement, while the second deals mainly with carbonised plant macroremains that were intentionally added by the dwellers to reinforce the large clayey object. Comparing plant remains that were preserved under various tapho- nomic conditions and collected by different sampling methods is important, because they can present dif- ferent sources of evidence (Jones 1991; Tolar 2016. 46, 56–59, 62–65, 142–148, 166). Material and methods Two methodologically different archaeobotanical in- vestigations from the same archaeological site (i.e. Stare gmajne) and period (the latest settlement phase) are presented and compared. The first inclu- des an analysis of the surface sediment samples taken systematically from the trench excavated in 2007. The second includes an investigation of the material from the broken loom-weight, called judgement sam- pling. Therefore, two sampling methods, surface and judgement (see e.g., Jacomet, Kreuz 1999; Tolar 2016.62–65) were applied and compared. Surface sampling from the trench From the Stare gmajne trench in 2007 (Fig. 1) sur- face sediment samples of approx. 1 litre of sediment per 1 depth/cut (see Fig. 1b) from the cultural layer were collected. Horizontally, it was sampled from three randomly chosen square metres (i.e. quadrants 1, 9 and 13) (Fig. 1a). The 46cm thick cultural layer was divided vertically into 8 depths/cuts of approx. 5cm (Fig. 1b). Depths/ cuts 4 and 5 pertain to the latest (i.e. the youngest) settlement phase, while depths/cuts 7 and 8 pertain to the earliest (i.e. the oldest) phase. Altogether, 15 samples with a total volume of some 9 litres were analysed. The samples were first washed and wet sieved, as proposed for waterlogged sedi- ments (see e.g., Hosch, Zibulski 2003; Tolar et al. 2010). Sieves with 2mm and 0.355mm mesh sizes were used. The collected waterlogged organic frac- tions were stored (and kept) in water and at low temperatures. Prior to the analysis, random sub-sam- pling was necessary, especially of the small (i.e. 0.355mm) fractions, because the samples were very rich in macroremains. In addition to whole seeds/ fruits and grains, grain fragments with embryo ends, glume bases, rachis fragments, fragments of seeds/ fruits with the base and flax (Linum) capsule frag- ments with a tip larger than 3mm were sorted out and counted. A Leica MZ75 stereomicroscope with 6.3 up to 50 magnification was used for the exam- ination. For identification, the reference collections of IPAS, Basel and Institute of Archaeology ZRC SAZU, as well as special literature (Berggren 1969; 1981; Anderberg 1994; Cappers et al. 2006; Jaco- met 2006) were consulted. Judgement sampling from the loom-weight A special and very important find, a loom-weight 22.5cm high and 14.4cm wide, was discovered in 2006. During the excavations, the bottom of the Fig. 1. The trench in 2007. a horizontally and b vertically (drawing T. Koro∏ec). Comparing different sampling methods in order to reconstruct plant economies at the Eneolithic lake dwelling site Stare gmajne ... 415 weight broke off and its incompletely burnt, clayey and waterlogged interior became visible. Its outer parts were burnt, but the interior was full of water- logged clayey material (Fig. 2) – original material for making larger clayey objects. The inner, clayey and waterlogged parts were re- moved from the weight (Fig. 2b). In total, almost 1 litre of clayey sediment was obtained and analysed. It was very carefully wet sieved through two sieves with standard mesh sizes (2 and 0.355mm). Both organic fractions were wet stored and examined un- der a stereomicroscope at up to 50 magnification(s). Identifications, type of remains, state of preserva- tion and counts were noted. A careful morphologi- cal study of different cereals remains (mainly chaff) was made and the proportions of different cereal types and crop species were calculated. Based on this, an estimate of the clay-straw ratio used for mak- ing the clayey objects was made (Tolar et al. 2016). The main aim of the present paper is to summarise and compare the archaeobotanical results from two contexts (surface sampling from the trench and judgement sampling from the loom-weight) from the same site and period (i.e. the latest phase of pile dwelling site Stare gmajne, dendro dated to c. 3160– 3100±14 cal BC) in order to reconstruct the plant economy at the site, especially of cereals. Results Surface sampling from the latest cultural layer of the Stare gmajne site The concentrations of the identified plant macrore- mains averaged 1572 remains per 1 litre of sedi- ment; 95.4% of the identifiable plant macroremains were preserved in an uncarbonised state, i.e. water- logged. A total of 38 taxa were identified, 5 of which were cultivated: emmer (Triticum dicoccum), einkorn (Triticum monococcum), barley (Hordeum vulga- re), poppy (Papaver somniferum), and flax (Linum usitatissimum) (Fig. 3). While the remains of poppy and flax were mainly represented by the remains of seeds/fruits (e.g., Figs. 3d, e), the cereal macrore- mains were mainly represented by chaff remains (e.g., Figs. 3a-c). Only 8 cereal grains (of all three taxa) were sorted out. The concentrations of cereal chaff remains (129 per 1 litre of sediment) are nor- mal for waterlogged cultural layers (or even lower; e.g., for the earliest settlement phase of Stare gmaj- ne site, the average concentration of cereal macro- remains is 652). They comprise general waste around the site and are therefore not concentrated in a par- ticular feature. Another 7 taxa were presumably collected for human consumption: turnip (Brassica rapa), acorn (Quer- cus sp.), hazelnut (Corylus avellana), water chestnut (Trapa natans), apple/pear (Maloideae), strawber- ry (Fragaria vesca), and blackberry (Rubus fruti- cosus). Surface sediment samples also include the remains of the wild (i.e. not nutritive) plant taxa: 4 water plants, 9 lakeshore plants, 6 field weed and ruder- al plants, 6 woodland plants, which give an insight into the ecological conditions at the site in the time of the pile dwellers. The settlement was located near the lake; the dwellers also exploited the surround- ing woodland for pasturing, gathering and tree felling (for details from both the latest and the ear- liest settlement phases, see Tolar et al. 2011). Judgement sampling from the Stare gmajne loom-weight The density of botanical macroremains was high: in the less than 1 litre of clayey material obtained Fig. 2. Incompletely burnt pyramid-shaped 1.5kg heavy clayey loom-weight was 22.2cm high and up to 14.4cm wide. During excavations (a) and du- ring cleaning in the lab (b) (photo a M. Turk and b T. Tolar). a b Tja[a Tolar, Anton Velu[;ek 416 from the loom-weight, a total of 1950 identifiable plant ma- croremains were sorted and counted. They were all pre- served in a carbonised or se- mi-carbonised state. Among them, more than 1800 very well preserved and easily identifiable cereal by-prod- ucts, i.e. chaff (Fig. 4) and 22 cereal grains were identified. The clay-organic material (mainly chaff) ratio was eas- ily determined. It is at least 93:7 (i.e. 93% of clayey mate- rial and at least 7% of cereal by-products) (for details, see Tolar et al. 2016). As well as 3 cereal taxa, em- mer (Triticum dicoccum), einkorn (Triticum monococ- cum) and barley (Hordeum vulgare), another 9 taxa were identified: poppy (Papaver somniferum), oak (Quercus sp.), brome grass (Bromus sp.), grasses (Poaceae), cf. millet (Panicum miliaceum), sedges (Cyperaceae), goosefoot (Chenopodium al- bum), moth mullein (Verbascum blattaria), and St John’s wort (Hypericum perforatum). In total, the 64 seeds/fruits of these ‘non-cereal’ taxa are not re- presentative enough to enable interpretation of the gathering economy or ecological conditions at the site. An experiment was conducted to learn why different processes occurred when the plant remains were de- posited in the loom-weight, i.e. mostly carbonised in waterlogged clayey material. Dry plant material (hay) was added to the clay from which two objects shaped similarly to the Stare gmajne loom-weight were made. As well as tempering, it became clear that the ad- ded plant material allowed very fine and easier mould- ing and forming of the raw clay. Two objects were made, each of them burnt (baked) on an open fire with hot coals for different times. The added plant remains in the object that was burnt for a longer time (i.e. 3 hours and 35 minutes) were completely charred and highly re- duced, while the plant remains in the second (that was burnt for 2 hours less) were differently preserv- ed, i.e. mostly in a carbonised and semi-carbonised state, and some not carbonised. Discussion Comparison between the surface and judge- ment sampled material Although in both cases of sampling, the researched material was waterlogged and clayey, the state of Fig. 3. The most important cultivar macroremains from the Stare gmaj- ne site, surface sampling: a emmer spikelet fork carbonised and uncarbo- nised; b einkorn spikelet fork carbonised and uncarbonised glume base; c barley rachis fragments carbonised and uncarbonised; d uncarbonised poppy seed; e uncarbonised flax seed; f carbonised barley grains (photos a-e T. Tolar, f D. Valoh). Fig. 4. Most frequent macroremains from the loom-weight cereal by-pro- ducts. a emmer spikelet forks; b glume bases; c barley rachis fragments (photo D. Valoh). preservation of the plant macroremains between the two compared contexts varies. Some 95.4% of macroremains from the surface cultural layer were preserved in an uncarbonised, i.e. waterlogged state (Fig. 3), while the plant remains from the loom- weight are all preserved in carbonised (71%) or at least in a semi-carbonised (29%) state (Fig. 4). Table 1 presents some basic differences between the surface (S) and the judgement (J) sampled material. Lower concentration of plant macroremains in the sediment samples from the cultural layer (S in Tab. 1) in comparison to the judgement sampled materi- al from the loom-weight (J in Tab. 1) is evidence of intentionally added (or used) plant macroremains (especially of cereals) in the judgement sample. Evi- dently, the greater diversity of plant taxa in the sur- face samples in comparison to the judgement sam- ple additionally indicates the intentional use of care- fully selected cereal by-products for tempering the large clayey object. The plant spectra between the compared contexts differs considerably. Samples from the cultural layer contain remains of diverse plants (38 taxa altoge- ther: cultivated, gathered and wild). Most of the re- mains are not carbonised, i.e. waterlogged. On the other hand, the sample from the loom-weight con- sists mainly (96%) of 3 cultivar species: emmer, ein- korn and barley, preserved in a semi-carbonised or fully carbonised state; these macroremains (espe- cially chaff) were intentionally added to the clay in order to obtain well-tempered material for making quite a large loom-weight. As the judgement sampled material contains evi- dently carefully selected and charred cereal by-pro- ducts, the only possible comparison between the two differently sampled and preserved materials is the importance of cereal species for the plant economy at the Stare gmajne site. Despite the different preser- vation conditions (waterlogged vs. charred) in both samples (surface and judgement) cereal by-products (i.e. chaff) prevail over cereal grains. Therefore, the grain units (i.e. 1 grain = 1 spikelet fork = 2 glume ba- ses = 1 barley rachis frg.) in 1 litre of sediment sam- ple of both investigated materials (surface-S and judgement-J) were compar- ed (Tab. 2). The only diffe- rence between the two is the preservation state: sur- Comparing different sampling methods in order to reconstruct plant economies at the Eneolithic lake dwelling site Stare gmajne ... 417 face – waterlogged chaff vs. judgement – charred chaff. Cereal macroremains (especially by-products) are obviously concentrated in the material from the loom- weight (1412-J : 526-S). It seems that the pro- portions of glume wheat-GW (i.e. emmer and ein- korn) remains in the loom-weight are higher than those of barley rachis fragments-B (949-GW : 463-B). In contrast, barley grain units in the wet soil surface samples from the cultural layer prevail over glume wheat grain units (387-B : 139-GW). Was barley more a popular crop species than glume wheats? Did the inhabitants deliberately choose glume wheat by-pro- ducts (although they were in a minority) for tem- pering? Data from a single loom-weight and the only and first appropriately investigated surface sediment samples from Slovenian pile-dwelling sites are not sufficient for definite conclusions. More archaeob- otanical studies at Ljubljansko barje pile-dwelling sites should be made to answer the two questions posed above. At the moment, we can only assume that different taphonomical processes are the most likely principal reason for such a result. Carbonised plant material from the loom-weight could contain more glume wheat by-products (i.e. glume bases and spikelet forks of emmer and einkorn) merely because of the taphonomy. The inhabitants may have simply used the available chaff without selec- tion, but differential carbonisation and destruction rates of different plant types (e.g., Bowman 1966; Hillman 1981; Boardman, Jones 1990; see also ex- perimental work in this paper and Tolar et al. 2016) that were added to the clay may have played the main role. This could be one of the reasons for the higher proportions of glume wheat over barley by- products preserved in the incompletely burnt loom- weight. Tempering with plant macroremains The clay which was used by the dwellers to make the weight was tempered with probably uncarbo- vol. of the vol. of organic no. of conc. per no. of no. of conc. of sediment fractions ident. plant 1 litre of cultivar gathered cultivar (ml) (ml) remains sediment taxa taxa remains S 3200 420 1583 495 5 7 141 J ∏ 1000 65 1950 π 1950 4 1 π 1875 Tab. 1. Some of the basic archaeobotanical results for surface sediment sam- ples S and judgement sample from the loom-weight- J. Both sediments were waterlogged preserved and represent the same site and period (latest settle- ment phase). Tja[a Tolar, Anton Velu[;ek 418 nised cereal chaff to reinforce it (the experi- mental work in this paper confirms this). It has already been proven that tempering was practised, not necessarily for loom-weights, at earlier settlements throughout Europe (e.g., Hopf 1977; 1980; Borojevi≤ 2006; Henn et al. 2015). Ethnographic evidence from present- day societies as well as the archaeobotanical evidence of past societies indicate that by-pro- ducts produced during cereal processing (i.e. chaff, straw and weed seeds, as well as some grains; e.g., Hillman 1981; 1984a) were used as fuel, fodder or temper (Watson 1979; van der Veen 1999; Henn et al. 2015). Examples of the use of cereal by- products for tempering in the late Neolithic have been found in plant impressions at many European sites (e.g., Hopf 1977; 1980; Bakels 1984; Borojevi≠ 2006). Imprints of wheat chaff and culm fragments of wild grasses on daub and ceramics (e.g., Kohler- Schneider 2007; Borojevi≤ 2006) are proof of this. It is very difficult both to precisely identify the plant material used and define its proportions, since there are no techniques to identify the actual quantity of straw and chaff used, because plant remains or even just their imprints are preserved in burnt and dried pottery or daub (Henn et al. 2015). Many attempts have been made to calculate this ratio (Ginder 1996; Borojevi≤ 2006; Henn et al. 2015), but the results have never been straightforward. The unburnt orig- inal material for making the clayey object from the Stare gmajne site gives us an opportunity to calcu- late this ratio. In less than 1 litre of waterlogged se- diment from the loom-weight, 65ml of organic frac- tions, mainly cereal by-products, were caught in the sieves. Some 1950 plant macroremains were sorted, identified and counted. Cereal by-products of bar- ley, emmer and einkorn predominate; in total, 1412 grain units were counted. The clay-organic material (mainly chaff) ratio is at least 93 : 7 (i.e. 93% of clay- ey material and at least 7% of cereal by-products). Conclusions The results of the plant macroremain studies of the Eneolithic lakeshore settlement Stare gmajne at Ljub- ljansko barje are presented. Archaeobotanical mate- rial was collected with two different sampling me- thods: 1) systematic surface sampling from the cultu- ral layer, and 2) judgement sampling from the in- completely burnt large loom-weight. When compar- ing the results, the preservation (taphonomy) and the spectra of plant macroremains diverse greatly, so the results of both studies yield different, but fa- vourable conclusions. The surface sampled sediment from the cultural la- yer gives an insight into the plant economy and nu- tritional habits of the settlers, as well as an insight into the environment and ecological conditions on and around the site. Six crop taxa were recorded. Plants that had been gathered from the woodland, woodland edges and the lake were also important for nutrition. Other non-nutritive seeds/fruits re- mains of wild flora indicate that the settlement was located close to the lake or a slow-flowing river. Marshy or lakeshore plant species and the ruderals grew nearby or even within the settlement. The re- mains of woodland plants and plants from wood- land edges suggest that the settlers exploited the nearby forest for gathering food, felling trees, pastu- ring animals and hunting. The judgement sampled sediment from the loom- weight deals primarily with carbonised and semi-car- bonised cereal macroremains (mostly chaff) that were preserved inside the loom-weight and were intentio- nally added to the clay to reinforce the large clayey object. This gives a detailed insight into the cereal cul- tivation and crop processing habits of the settlers. The deliberate use of cereal by-products was confirmed. The clay-cereal chaff ratio (93:7) was established. It was possible to compare both studies with regard to the cereal economy. The cereal by-products, which were preserved in two different ways (not carbo- nised in surface samples vs. carbonised in the loom- weight), confirm the cultivation of the main crops: emmer, einkorn, and barley. The greater importance of barley is suggested by the surface samples, but the judgement sample does not confirm this. The dif- ferential carbonisation and destruction rates of diffe- rent plant types when exposed to heat (e.g., Board- man, Jones 1990; see the experiment) prevent us from establishing which crop species predominated at the Stare gmajne site. The investigation of the surface samples yields ad- ditional information about the plant economy. The emmer einkorn emmer\einkorn barley (Triticum (Triticum (Triticum (Hordeum Total dicoccum) monococcum) di\monococcum) vulgare) S 67 72 387 526 J 482 193 274 463 1412 Tab. 2. Numbers (concentrations) of grain units in 1 litre of sediment for 3 cereal taxa. S surface samples from the cultural layer in which uncarbonised cereal chaff predo- minate; J judgement sample from the loom-weight in which carbonised cereal chaff predominate. Comparing different sampling methods in order to reconstruct plant economies at the Eneolithic lake dwelling site Stare gmajne ... 419 settlers’ activities and plant gathering, pasturing and fodder collection had an impact on the vegetation in the vicinity of the settlement (also e.g., Andri≠ et. al. 2008; Andri≠ in prep.). The judgement sample provides supplementary in- formation about crop husbandry practices, the de- velopment stage and the inventiveness of the Ene- olithic settlers. According to Marijke van der Veen (1999), the use of chaff can be linked to various scales of production and organisation in the agri- cultural system. 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