Acta Dermatovenerol APA Acta Dermatovenerologica Alpina, Pannonica et Adriatica 2015;24:21-23 doi: 10.15570/actaapa.2015.7 Genome announcement: complete genome sequence of a novel Mupapillomavirus, HPV204 Boštjan J. Kocjan1, Anja Šterbenc1, Lea Hošnjak1, Diego Chouhy2, Elisa Bolatti2, Adriana A. Giri2, Mario Poljak1 13 Abstract Human papillomaviruses (HPVs) are small, non-enveloped viruses with a circular double-stranded DNA genome, etiologically associated with various benign and malignant neoplasms of the skin and mucosa. As of May 30, 2015, 201 different HPV types had been completely sequenced and officially recognized and divided into five PV-genera: Alpha-, Beta-, Gamma-, Mu-, and Nupapil-lomavirus. The Mupapillomavirus genus currently consists of only two HPV types: HPV1 and HPV63, identified in 1980 and 1993, respectively, both associated with sporadic cases of cutaneous warts. In this preliminary study, we announce the complete genome sequence of a novel HPV type, now officially recognized as HPV204. Based on preliminary data, the genome of HPV204 comprises a total of 7,227 bp and contains five early open reading frames (E1, E2, E4, E6, and E7) and two late ORFs (L1 and L2). No E5 ORF could be identified. Preliminary HPV204 clusters to the Mu-PV genus, species Mu-3. Keywords: human papillomavirus, HPV, HPV204, Mupapillomavirus, genomic characterization Received: 10 April 2015 | Returned for modification: 26 April 2015 | Accepted: 12 May 2015 Human papillomaviruses (HPVs) are a large family of small, genetically diverse DNA viruses that can cause benign and malignant proliferations of the skin and mucosal epithelia (1, 2). HPVs are taxonomically classified into genera, species, and types based on sequence similarities of the highly conserved Li genomic region. A novel HPV type is recognized as such when its genome has been fully cloned and deposited to the International HPV Reference Center (www.hpvcenter.se) and its Li nucleotide (nt) sequence shows less than 90% identity to any other known HPV type (1, 3). As of May 30, 2015, 201 different HPV types belonging to 49 viral species had been completely sequenced and officially recognized and divided into five papillomaviruses (PV)-genera: Alphapapillomavirus (Alpha-PV), Betapapillomavirus (Beta-PV), Gammapapillomavirus (Gamma-PV), Mupapillomavirus (Mu-PV), and Nupapillomavirus (Nu-PV) (4). Several additional HPVs have been completely sequenced, mainly using next-generation sequencing, but are not yet officially recognized (5). These are listed in the PapillomaVirus Episteme (PaVE) database, together with the sequences of animal PV genomes (5). The Mu-PV genus currently consists of only two cutaneotrophic HPV types: HPV1 (species Mu-1) and HPV63 (species Mu-2), which are etiologically associated with sporadic cases of skin warts (6). HPV1 was identified in 1980 in tissue specimens of deep plantar warts (7) and fully sequenced 2 years later (8). HPV63 was identified and fully sequenced in 1993 from a tissue specimen of a kera-totic lesion (9). In recent years, the number of Gamma-PVs has been growing most rapidly, with 81 completely sequenced types to date, followed by Alpha- and Beta-PV genera, which consist of 65 and 51 recognized types, respectively (4, 10). In contrast, only a single putatively novel Mu-PV sequence has been identified in recent years (11), although several modern molecular approaches for virus detection, including metagenomic sequencing, were used (4). We report here the preliminary genome sequence of a novel Mu-PV type HPV204. A partial 200-bp L1 gene nt sequence (ENA accession number: FJ947082) similar to both Mu-PVs (HPV1 and HPV63) was origi- nally identified in 2009 using FAP primers and single tube "hanging droplet" PCR (11). A complete viral genome was amplified in 2014 using inverse long-range PCR with primer pair FJ947082-LR-F (5'-GGTATGGGCATTAAGAGGTTTA-3', nt 5,484-5,505) and FJ947082-LR-R (5'-AAGCGTTCTGTTTCAGGATTAA-3', nt 5,4835,462) and the Platinum Taq DNA Polymerase High Fidelity Kit (In-vitrogen, Carlsbad, CA). The resulting amplicon of approximately 8 kbp was gel-purified, cloned into a plasmid vector using TOPO XL PCR Cloning Kit (Invitrogen), and sequenced on both strands at Microsynth AG (Balgach, Switzerland) using the primer-walking strategy. The nt sequence of the complete viral genome was assembled using Vector NTI Advance 11 software package (Invitrogen). PV-specific open reading frames (ORFs) were determined using the ORF Finder Tool (http://www.ncbi.nlm.nih.gov/gorf/ gorf.html). Preliminary phylogenetic relationships were inferred by Bayesian analysis, based on the entire L1 gene nt sequence of HPV204 and 61 officially recognized HPV types from the Alpha-, Beta-, Gamma-, Mu-, and Nu-PV genera. Markov Chain Monte Carlo simulations were performed on 107 generations, sampling one state every 1,000 generations, with a burn-in of 10%. The evolutionary substitution model was set as rtREV + r + I. A reference clone, containing the complete genome of HPV204, was deposited in the HPV Reference Center at the Karolinska Institute in Sweden in October 2014, where its nucleotide sequence was confirmed and the type officially designated in January 2015 (http://www.hpvcenter.se/html/refclones.html). Based on preliminary data, the genome of HPV204 comprises a total of 7,227 bp and contains five early ORFs (E1, E2, E4, E6, and E7), coding proteins involved in viral replication, transcription, and transformation, and two late ORFs (L1 and L2), coding proteins of the capsid. No E5 ORF that is present in some PVs (12) could be identified. The non-coding long control region (LCR) that contains elements responsible for controlling transcription and replication of the viral genome was identified between the L1 and E6 ORFs. Preliminarily, with an L1 nt sequence identity of 66.3% and 66.7% to HPV1 and HPV63, respectively, HPV204 is •institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. 2Virology Area, School of Biochemistry and Pharmaceutical Sciences, Rosario National University, Rosario, Argentina. h Corresponding author: mano.poljak@mf.um-lj.si 2 B. J. Kocjan et al. Acta Dermatovenerol APA | 2015;24:21-23 taxonomically classified as a novel Mu-PV type in a new species Mu-3. As shown in Figure 1, our preliminary phylogenetic analysis of HPV204 and 61 HPV Li nt sequences revealed that HPV204 clusters with HPVi and HPV63, suggesting that the novel virus is most probably an indisputable member of the Mu-PV genus. In conclusion, the identification of HPV204 expands the current knowledge of the genetic diversity of the Mu-PV genus and provides further information for the development of molecular tools for identifying and isolating novel Mu-PVs. The detailed genomic characterization of HPV204, its tissue tropism, and potential clinical significance are currently under investigation and will be reported soon. Nucleotide sequence accession number. The complete genome sequence of HPV204 is available in the ENA, GenBank, and DDBJ databases under the following accession number: KP769769. Figure 1 | Phylogenetic position of HPV204 (KP769769) in the Mu-PV genus. The numbers at the internal nodes represent Bayesian posterior probability values. 22 Acta Dermatovenerol APA | 2015;24:21-23 Novel Mupaplllomavlrus Acknowledgements This work is result of PhD research and is financially supported by the Slovenian Research Agency (ARRS), which funded A§ through the Young Researcher Training Program, and in part by Slovenian-Argentinian Bilateral Project BI-AR-15-17-005 and by the European Commission's FP7 Program CoheaHr project (grant number: 603019). References 1. Bernard HU, Burk RD, Chen Z, van Doorslaer K, zur Hausen H, de Villiers EM. Classification of papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendments. Virology. 2010;401:70-9. 2. Cubie HA. Diseases associated with human papillomavirus infection. Virology. 2013;445:21-34. 3. de Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H. Classification of papillomaviruses. Virology. 2004;324:17-27. 4. Kocjan BJ, Bzhalava D, Forslund O, Dillner J, Poljak M. 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