Analysis of cord blood units donated to the Slovenian cord blood bank
Analiza popkovnične krvi, darovane v slovensko banko popkovnične krvi

slovenian

2009

volume 78, issue 9

Antigens, Cd34   Blood Banks   Cell Count   Fetal Blood   Flow Cytometry   Hematopoietic Stem Cells   Umbilical Arteries

Slovensko zdravniško društvo
(Obvezni izvod spletne publikacije)

Background Umbilical cord blood (CB) as well as bone marrow has been used to treat a variety of haematopoietic disorders. The most important factors that influence the outcome of haematopoietic stem cell transplantations are the HLAmatch and the number of transplanted nucleated cells. The aim of our research was to analyse CB units donated to the Slovenian cord blood bank. Methods The concentrations of nucleated cells (NC) and haematopoietic stem cells (CD34+ cells) in CB were measured with a haematology analyser and flow cytometry. Growth of colonyforming cells (CFC) was determined in cell culture.The results were statistically evaluated using the Pearsons correlation analysis. Results The median NC concentration in CB was 10.6 X 10 9/L (range: 4.07-26.2 x 10 9/L), and the median number of NC was 721 X 10 6 (221 - 2157 X 10 6). The median collected volume of CB including 21 mL of anticoagulant was 73 mL (32 - 150 mL). The median concentrations of CD34+ cells and CFC were 35.5/micoL (6.6 - 142.8/microL) and 17.7/microL (2.2 - 55.9/microL), respectively. The ratio of CD34+/CFC was 1.9 (range, 1.0 - 13.6), with a statistically significant positive correlation coefficient of 0.87. While there was a good correlation between the concentrations of NC and CD34+ cells, no correlation was observed between those concentrationsand the collected volume of CB. Conclusions Of donated CB units, 46 % contained more than 750 X 10 6 nucleated cells and fulfilled the criteria for CB storage in our bank.

18.05.2010