Steroid hydroxylating enzymes in the fungus Rhizopus nigricans are induced by progesterone and by some other steroids. It is known that in higher organisms steroids exert their nongenomic action via steroid binding proteins located inthe plasma membrane of the cells, thus our aim was to detect progesterone binding sites in R. nigricans plasma membrane. In this report membrane receptors were identified by two independent methods, analysis of progesteronebinding to plasma membrane fraction by competitive binding assay using š3Hđ- and š1Hđ progesterone (ECSO=55 nM) and in situ binding of fluorescein isothiocyanate-coupled cell impermeant progesterone conjugate to fungal protoplasts, detected by fluorescence microscopy.

Rights

URN

URN:NBN:SI:DOC-3FAEZDPN

COBISSID

17399257

Added

16.09.2009

Metadata

Citation

APA:

Jeraj, Nataša, Romih, Rok, Lenasi, Helena, Breskvar, Katja (2003). In situ detection of progesterone binding sites in the plasma membrane of the filamentous fungus Rhizopus nigricans. Acta chimica slovenica, volume 50, issue 4, str. 757-762. URN:NBN:SI:DOC-3FAEZDPN from http://www.dlib.si

MLA:

Jeraj, Nataša, Romih, Rok, Lenasi, Helena, Breskvar, Katja. "In situ detection of progesterone binding sites in the plasma membrane of the filamentous fungus Rhizopus nigricans." Acta chimica slovenica volume 50. issue 4 (2003) str. 757-762.
<http://www.dlib.si/?URN=URN:NBN:SI:DOC-3FAEZDPN>

Acta chimica slovenica
1993-
(Assembly record)

In situ detection of progesterone binding sites in the plasma membrane of the filamentous fungus Rhizopus nigricansIn situ detekcija vezavnih mest za progesteron v plazemski membrani filamentozne glive Rhizopus nigricans

In situ detection of progesterone binding sites in the plasma membrane of the filamentous fungus Rhizopus nigricans
In situ detekcija vezavnih mest za progesteron v plazemski membrani filamentozne glive Rhizopus nigricans