NATURA SLOVENIAE 26(2): 21-33 Prejeto / Received: 26. 11. 2024 SHORT COMMUNICATION Sprejeto / Accepted: 20. 12. 2024 DOI: 10.14720/ns.26.2.21-33 Biotehniška fakulteta Univerze v Ljubljani in Nacionalni inštitut za biologijo, Ljubljana, 2024 First records of two slug species, Limax conemenosi (O. Boettger, 1882) and Limax dacampi (Menegazzi, 1855) (Gastropoda: Limacidae) in Slovenia Maj KASTELIC, Behare REXHEPI University of Ljubljana, Biotechnical Faculty, Department of Biology, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia; E-mail: mk09679@student.uni-lj.si Abstract. In Slovenia, 27 species of slugs have been confirmed so far. Distributional data for two species from the genus Limax new for the country are presented herewith. Limax conemenosi was found for the first time in Slovenia at Nova Gora near Krško, whereas Limax dacampi was first recorded in the village of Osp and recently in the village of Abitanti. Species identities were assessed on the basis of external morphological features and diagnostic DNA sequences of cytochrome c oxidase subunit I. Subsequently, the new number of slug species confirmed in Slovenia is 29. Key words: Gastropoda, slugs, Limax, DNA sequences, distribution, Slovenia Izvleček. Prvi podatki o dveh vrstah golih polžev, pikastem slinarju Limax conemenosi (O. Boettger, 1882) in rdečem slinarju Limax dacampi (Menegazzi, 1855) (Gastropoda: Limacidae) v Sloveniji – Do zdaj je bilo v Sloveniji potrjenih 27 vrst golih polžev. V prispevku predstavljamo podatke o razširjenosti dveh vrst iz rodu Limax, novih za državo. Pikasti slinar Limax conemenosi je bil v Sloveniji prvič najden v Novi Gori v bližini Krškega, rdeči slinar Limax dacampi pa je bil prvič zabeležen v Ospu in nedavno v Abitantih. Vrsti smo potrdili na podlagi zunanjih morfoloških značilnosti in pridobljenih DNK-sekvenc podenote 1 citokrom C oksidaze. Tako je novo število potrjenih vrst golih polžev v Sloveniji zdaj 29. Ključne besede: Gastropoda, goli polži, Limax, DNA-sekvence, razširjenost, Slovenija Introduction Slugs are an understudied group of molluscs in Slovenia, with the first comprehensive paper on slugs published in 2002 (Vaupotič & Velkovrh 2002). The authors published a revised list of species with maps of distributions, reporting on 27 slug species for Slovenia. They collected data from older publications and complemented them with additional species records with the study of approximately 600 samples of slugs from three collections, including six new species for Slovenia (Vaupotič & Velkovrh 2002). There are five different families of slugs in Slovenia – Milacidae, Limacidae, Boettgerilidae, Agriolimacidae and Arionidae (Vaupotič & Velkovrh 2002). The Limacidae family is represented by five species, two of them belonging to the genus Limax. 22 Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION NATURA SLOVENIAE 26(2): 21-33 One of them is Limax maximus (Linnaeus, 1758), known for a distinct pattern of dark blots and dots on the upper side of the foot and mantle (Welter-Schultes 2012). The second one is Limax cinereoniger (Wolf, 1803), a species with highly variable dark colour patterns and a distinctive sole of the foot with two dark longitudinal bands on the sides (Welter-Schultes 2012) that make it easily recognizable. Both species have wide distribution in Slovenia (Vaupotič & Velkovrh 2002). In this contribution, we report on new data for two species, Limax conemenosi (O. Boettger, 1882) and Limax dacampi (Menegazzi, 1885), new for Slovenia. Materials and methods Field work Discoveries of both species are the result of accidental findings, with targeted field studies following the first discoveries. At the site at Nova Gora near Krško (Figs. 1, 3) slugs were first found accidentally under a wooden board. We searched for the species again twice later on, during the day by flipping wooden material, and in the night when slugs are active (Salvador & Tomotani 2024) by slow inspection of the terrain. We collected the biggest individual for subsequent determination. First discoveries of another species of slugs from the sites in southwestern Slovenia (Fig. 1) were also accidental. Slugs were found for the first time in the night time in 2011 at a site near Osp. Recently we found the species at another site in Abitanti (Figs. 1, 5) during our night fieldwork searching for amphibians. We observed slugs moving around in the grass and on the rock drywalls and collected one big individual for determination. Another search during the day by flipping rocks and wooden material wasn’t successful. Morphological determinations We determined both collected individuals still alive on the basis of the external morphological features described by Welter-Schultes (Welter-Schultes 2012). Both individuals were photographed and released in the places of their discovery. Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION 23 NATURA SLOVENIAE 26(2): 21-33 Figure 1. Distribution map of two newly discovered Limax species for Slovenia. Circles represent sites of discovery of Limax dacampi in southwestern Slovenia. Osp is marked with number 2 and Abitanti with number 3. Rhombus marked with number 1 represents site of discovery of Limax conemenosi in Nova Gora. Slika 1. Zemljevid razširjenosti dveh novoodkritih vrst slinarjev (Limax) v Sloveniji. Kroga ponazarjata mesti najdb rdečega slinarja Limax dacampi v jugozahodni Sloveniji. Osp je označen s številko 2 in Abitanti s številko 3. Romb, označen s številko 1, ponazarja mesto najdbe pikastega slinarja Limax conemenosi v Novi Gori. Molecular determinations In order to confirm both morphological determinations, we sequenced and analysed DNA sequences of cytochrome c oxidase subunit I of collected individuals. We isolated DNA from a small piece of tissue from the tip of the tail, using MagMAX DNA Multi-sample Kit (Thermo Fisher Scientific Inc., United states), following the instructions of the producer. We amplified a 650 bp fragment of the mitochondrial cytochrome oxidase I (COI) gene using the KAPA2G Robust PCR Kit (Sigma-Aldrich, USA) and primers LCO1490 and HCO2198 (Folmer et al. 1994). The PCR products were purified with Exonuclease I and FastAP (Thermo Fisher Scientific, USA) and sequenced bidirectionally by Macrogen Europe (Amsterdam, Netherlands) using the same primers. Chromatograms were assembled and edited in Geneious Prime 2023.2.1. The sequences were aligned with sequences of the Limax species that are morphologically similar and hence mistakable for the species we found and are also available in Genbank. We constructed a maximum likelihood phylogenetic tree using IQ-TREE with default settings (Minh et al. 2020) to perform DNA barcoding and determining whether our samples match any of the available sequences. 24 Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION NATURA SLOVENIAE 26(2): 21-33 Results and discussion The data on two species of slugs, Limax conemenosi and Limax dacampi, indicate the first confirmation of their presence for Slovenia. Comments on Limax conemenosi findings Limax conemenosi is a cream or yellowish-coloured slug with numerous 1 mm big black dots equally distributed over the upper side of the mantle and the foot (Fig. 2). The sole is uniformly coloured (Welter-Schultes 2012). It lives in bush thickets and rocky open habitats, often as a synanthrope. It is less frequently found in forests (Wiktor 1996). Figure 2. Conemenos’ slug Limax conemenosi collected in Nova Gora on 6. 4. 2024. Photos are taken on one centimetre grid paper, showing external morphological features from different angles (photo: Maj Kastelic). Slika 2. Pikasti slinar Limax conemenosi, ujet v Novi Gori 6. 4. 2024. Fotografiran je na papirju s centimetrsko mrežo iz različnih zornih kotov, ki prikazujejo njegove zunanje morfološke značilnosti (foto: Maj Kastelic). Limax conemenosi probably originates from Greece, but is also found in Bulgaria, North Macedonia, Kosovo (Wiktor 1996), Albania (Dhora 2014) and Montenegro (Jovanović 1995). There are no official records from Bosnia and Herzegovina and Croatia (Wiktor 1996; Štamol 2010; Duda & Haring 2023), but photos of slugs on the citizen science platform iNaturalist that have external morphological features characteristic of L. conemenosi are from localities as far north as Croatian Istria (iNat 2024). In Serbia, there have been no official records of the species, even though the southernmost tip of the country is marked on the distribution map in the book European non-marine molluscs, a guide for species identification (Welter- Schultes 2012). Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION 25 NATURA SLOVENIAE 26(2): 21-33 The slug L. conemenosi was first found accidentally under a wooden board in the village of Nova Gora near Krško (WGS84 lat./long.: 45.953872 °N, 15.399901 °E; alt. 415 m) (Fig. 3) on 30. 3. 2024. We found two individuals. Figure 3. Habitat of Limax conemenosi in Nova Gora on 29. 9. 2024. All of the individuals were found in close proximity to the houses with several surrounding vineyards and extensive orchards (photo: Matej Kastelic). Slika 3. Habitat pikastega slinarja Limax conemenosi v Novi Gori 29. 9. 2024. Vsi osebki so bili najdeni v bližini hiš. Ob hišah je veliko vinogradov in ekstenzivnih sadovnjakov (foto: Matej Kastelic). The species was rediscovered on 6. 4. 2024 when six individuals were found during the day under wooden boards. The biggest one of them was collected for determination (Fig. 2). Three more individuals were observed on 7. 9. 2024 in the night time when they were active. The species identity was confirmed using CO1 sequences. The Nova Gora slug sequence, deposited in GenBank under accession number PQ676333, shows 99.11% similarity to sequence MT293865.1, 99.07% to MT293864.1 and 98.48% to sequence MT975672.1 (Tab. S1, Supp. Mat.). All three reference sequences are deposited as L. conemenosi. The two most similar sequences (MT293865.1 and MT293864.1) were obtained from juvenile slugs near Lake Volvi (Dimzas et al. 2020). These identifications relied solely on external morphology, as reproductive system data was unavailable. At the time, no L. conemenosi sequences were officially designated (Dimzas et al. 2020). The third sequence (MT975672.1) comes from a slug collected on Corfu by R. Anderson in 2018 (Giusti et al. 2021). The relationships between our sequence and the reference sequences are illustrated in the phylogenetic tree (Fig. 6). 26 Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION NATURA SLOVENIAE 26(2): 21-33 Table 1. External morphological characteristics of the collected individual determined as L. conemenosi (»specimen«) and morphologically similar species living in Europe. Descriptions of characteristics are taken from Welter-Schultes (2012). Grey background with text in bold means that a certain characteristic of a species is the same as of the collected individual. White background with normal text signifies that a certain characteristic of a species differs from the characteristic of the collected individual. NA on white background means that a certain description is not available. Among the species in the table, which are all morphologically similar, only L. maximus was known to have occurred in Slovenia so far. Tabela 1. Zunanje morfološke značilnosti preučevanega osebka določenega kot L. conemenosi (»specimen«) in morfološko podobnih vrst, ki živijo v Evropi. Opisi lastnosti so vzeti iz Welter-Schultes (2012). Sivo ozadje s poudarjenim besedilom pomeni, da je opisana lastnost enaka lastnosti preučevanega osebka. Belo ozadje z nepoudarjenim besedilom pomeni, da je opisna lastnost drugačna od lastnosti preučevanega osebka. NA na belem ozadju pomeni, da določen opis ni na voljo. Od vrst v tabeli, ki so si vse morfološko podobne, je bilo do sedaj v Sloveniji znano le pojavljanje vrste L. maximus. Outer morphological characteristics »specimen« Limax conemenosi Limax millipunctatus mantle size relatively short (1/4 of body, 30 mm) relatively short (1/4 of body, up to 22 mm) NA mantle shape rounded at posterior end NA mantle anteriorly rounded and pointed at posterior end, concentrically striated keel very short and not prominent very short (10 mm) and not prominent short and not very prominent tubercules elongated NA oval and not very prominent dorsum of the foot rounded and little darker than sides rounded and little darker than sides brown-reddish hue, coloured medial line in posterior section sides of the foot a little lighter than dorsum a little lighter than dorsum slightly less regular sole of the foot sole light, like the background colour sole light, like the background colour evenly white dots numerous small black dots with sharp edges numerous 1 mm large black dots with sharp edges numerous black dots pattern of dots regularly spotted, many dots regularly spotted evenly distributed colour light creamish to pinkgray dirty cream, yellowish or reddish grey greyish or yellowish with brown-reddish hue size 130 mm up to 120 mm 115–120 mm pneumostome without distinct margin, slightly posterior of the centre of the mantle 21–23 fine and fIattened wrinkles between medial line of dorsum and pneumostome without distinct margin, slightly posterior of the centre of the mantle tentacles without dots, bases of tentacles not approaching each other NA finely spotted, bases of tentacles not approaching each other mucus colourless colourless NA known distribution Nova Gora near Krško originally probably Greece, at present also Bulgaria to Albania Lombardia, N Apennines (cont.) Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION 27 NATURA SLOVENIAE 26(2): 21-33 Table 1. (cont.) Tabela 1. (nad.) Outer morphological characteristics Limax punctulatus Limax canapicianus Limax redii Limax maximus mantle size NA NA NA 30% of the body mantle shape NA rounded at posterior end NA pointed at posterior end keel light and extending over 1/2 of the dorsum length short and weakly lighter brown than dorsum NA clearly visible, 1/3 of body length tubercules elongated and irregular NA NA NA dorsum of the foot light greyish with yellowish hue light brown creamy or yellowish, sometimes with bluish hue ash-grey-cream or brownish, variable sides of the foot dots slightly less regular light brown creamy or yellowish, sometimes with bluish hue ash-grey-cream or brownish, variable sole of the foot evenly white and subdivided in 3 equally wide longitudinal zones evenly white NA uniformly cream- coloured dots numerous black dots (0.5 mm big on mantle) black, nearly rounded small black most slugs with large blots and variable mantle spots pattern of dots evenly distributed (12–14 dots in 3 to 4 longitudinal rows) NA scattered black dots, less dotted on mantle variable patterns of blots and dots colour light greyish with yellowish hue (lighter than L. millipunctatus) light brown creamy or yellowish, sometimes with bluish hue ash-grey-cream or brownish, variable size up to 130 mm up to 140 mm up to 150 (exceptionally 230 mm) up to 200 cm pneumostome darker margin, slightly posterior of the centre of the mantle in the mid section of the mantle NA 21–36 wrinkles between medial line of dorsum and pneumostome tentacles bases approaching each other NA grey NA mucus NA NA NA colourless known distribution N Italy, Bulgaria? NW Italy (Alpi Graie) N Italy (Como, Varese), S Switzerland (Ticino, W of Lago di Como) Originally S and W Europe, perhaps also N Africa, today widespread in Europe, Caucasus and abroad 28 Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION NATURA SLOVENIAE 26(2): 21-33 The specimen examined herewith best corresponds to description of L. conemenosi (Welter-Schultes 2012) (Tab. 1). Comments on Limax dacampi findings Limax dacampi is a red slug with dark colour bands at the sides or dark spots on the dorsum (Fig. 4). It grows up to 20 cm long and has a distinctive sole of the foot with two dark longitudinal bands on the sides as L. cinereoniger (Welter-Schultes 2012). It is known from different locations in Italy, where it was first described in 1885 at Lago di Garda by Menegazzi, even though new individuals were only rediscovered in type locality quite recently (in 2008) when mating was also documented (Falkner et al. 2008). It lives in different habitats, preferring forests and shrubs in lowlands (Welter-Schultes 2012). The species itself shows high morphological variability and is taxonomically problematic. It is probably a complex of different species (Falkner et al. 2008). It is known from different localities in Italy (Falkner et al. 2008; Rowson et al. 2014) as well as Switzerland, Croatia and the UK (Rowson et al. 2014). In the UK, it is only found in one location in North-east Yorkshire and has almost certainly been introduced from central Italy (Rowson et al. 2014). There is no record of L. dacampi in Slovenia in older publications (Wiktor 1996; Vaupotič & Velkovrh 2002). The species was first recorded in Slovenia on 11. 8. 2011, when Borut Kumar found L. dacampi in Osp. Identity of the slug was determined by Clemens M. Brandstetter (moderator of malacological part of forum Natura Mediteraneo, member of Die Gesellschaft zur Kartierung der Wirbellosen in Vorarlberg und Liechtenstein). The finding remained unpublished at the time. We found L. dacampi for the first time on a forest road near the village of Abitanti (WGS84 lat./long.: 45.436781 °N, 13.822295 °E; alt. 396 m) (Fig. 5) on 11. 5. 2024 during the night when slugs were active. We found eleven individuals and took pictures of every individual. We collected one of them for identification (Fig. 4). We revisited the site on 29. 8. 2024 in time of drought, but found no individuals. The identity of the species was confirmed based on CO1 sequences. Sequence of the slug from Abitanti is deposited in GenBank under accession number PQ676334 and is 99.84% identical to the sequence JX435840.1, 99.68% to the sequence JX435860.1, 99.66% to the sequence JX435861.1, 97.71% to the sequence JX435862.1 and 96.61% to sequences KF894382.1 and KF894384.1. All six corresponding sequences are deposited in GenBank as L. cf. dacampi. Two most similar sequences (JX435840.1 and JX435860.1) come from slugs from Croatian Istria (Nitz 2013). Two least similar sequences come from slugs from north-east Yorkshire (Rowson et al. 2014). Slug with most similar sequence was found in 1991 at Motovun (Nitz 2013) just approximately 11 km from where we found L. dacampi, which could mean that L. dacampi was present in Slovenia for quite some time, before it was first found. Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION 29 NATURA SLOVENIAE 26(2): 21-33 Figure 4. Limax dacampi collected at Abitanti on 11. 5. 2024. Photos are taken on one centimetre grid paper, showing external morphological features from different angles (photo: Maj Kastelic). Slika 4. Rdeči slinar Limax dacampi, ujet v Abitantih 11. 5. 2024. Fotografiran je na papirju s centimetrsko mrežo iz različnih zornih kotov, ki prikazujejo zunanje morfološke značilnosti (foto: Maj Kastelic). Figure 5. Habitat of Limax dacampi at Abitanti on 29. 8. 2024. Slugs were found on a forest road next to dry stonewalls (photo: Maj Kastelic). Slika 5. Habitat rdečega slinarja Limax dacampi v Abitantih 29. 8. 2024. Gole polže smo našli na kolovozu ob suhozidih (foto: Maj Kastelic). 30 Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION NATURA SLOVENIAE 26(2): 21-33 Figure 6. Phylogenetic tree including sequences of slugs examined herein (shown in green), morphologically similar species living in Europe and species from the Limacidae family living Slovenia. Limax millipunctatus, Limax canapicianus and Malacolimax mrazeki are not shown in the tree, since there are no available sequences. The numbers behind species name are accession numbers from GenBank. Numbers at nodes indicate bootstrap support. Sources of sequences are shown in Tab. S1 in Supplementary Material. Slika 6. Filogenetsko drevo s sekvencama preučevanih golih polžev (napisani z zeleno barvo), morfološko podobnih vrst, živečih v Evropi, in vrst iz družine slinarjev Limacidae, ki živijo v Sloveniji. Limax millipunctatus, Limax canapicianus in Malacolimax mrazeki na drevesu niso prikazani, ker njihove sekvence niso na voljo. Števila za vrstnimi imeni so dostopne kode sekvenc, objavljenih na strani GenBank. Številke na razvejitvah prikazujejo vrednost samovzorčenja (bootstrap support). Viri sekvenc so prikazani v Tab. S1 v Dodatnem materialu. 0.06 Limax_conemenosi_MT975672 Limax_cinereoniger_KF894353 Limax_cf._dacampi_KF894384 Limax_cf._dacampi_JX435861 Limax_cinereoniger_KF894305 Limax_cf._dacampi_JX435840 Limax_cinereoniger_FJ606463 Limax_conemenosi_MT293865 Limax_cinereoniger_FJ606461 Limax_cinereoniger_FJ606465 Limax_cinereoniger_KF894257 Limacus_flavus_ON123511 Limacus_flavus_OP270478 Limax_redii_JX435877.1 Limax_cinereoniger_KF894231 Limax_cinereoniger_KF894371 Limax_cinereoniger_KF894284 Limax_cf._dacampi_JX435862 Limax_cinereoniger_KF894347 Limax_punctulatus_ON123540 Limax_cinereoniger_FJ606459 Limax_cinereoniger_MT579458 Limax_cinereoniger_FJ606458 Limax_cinereoniger_KF894365 Limax_cinereoniger_FJ606460 Limacus_flavus_OP663291 Limax_cf._dacampi_KF894382 BA961_Limax_conemenosi Limax_cinereoniger_KF894339 Limax_cinereoniger_KF894380 Limax_cinereoniger_KF894220 Lehmannia_marginata_KF894288 Limax_cinereoniger_KF894295 Limacus_flavus_MT947692 Limax_conemenosi_MT293864 Limax_cinereoniger_FJ606462 Limax_punctulatus_ON123541 Limax_cinereoniger_KF894317 Limax_cf._dacampi_JX435860 Lehmannia_marginata_KF894298 Limax_cinereoniger_KF894227 Limax_cinereoniger_KF894232 Limax_cinereoniger_FJ606464 Limax_cinereoniger_KF894291 Limacus_flavus_OP663292 Limacus_flavus_ON123512 BA962_Limax_cf._dacampi 72 100 100 72 59 86 Maj KASTELIC & Behare REXHEPI: First records of two slug species ... / SHORT COMMUNICATION 31 NATURA SLOVENIAE 26(2): 21-33 By confirming the presence of both species in Slovenia, the number of Limax slug species in Slovenia has increased to four, and total number of known slug species to 29. The group remains severely understudied in Slovenia, and further research is needed to improve our knowledge. Additional studies are likely to uncover more previously unrecorded species for the country. Povzetek V kratki vesti so predstavljeni podatki o dveh prvih najdbah vrst iz rodu slinarjev Limax. Iz družine Limacidae je bilo do sedaj znanih pet vrst, od tega dve vrsti iz rodu Limax (Vaupotič & Velkovrh 2002). Rdečega slinarja Limax dacampi, ki izvira iz Italije (Rowson et al. 2014), je prvič našel Borut Kumar leta 2011 v Ospu, potrdil pa Clemens M. Brandstetter, vendar najdbe nista objavila. V Abitantih smo 28. 11. 2024 našli odrasle osebke vrste in enega izmed njih vzeli za determinacijo (Sl. 4). Pikastega slinarja Limax conemenosi, ki verjetno izvira iz Grčije (Welter-Schultes 2012), pa smo prvič odkrili 30. 3. 2024 v Novi Gori v bližini Krškega. Največjega izmed najdenih osebkov smo odvzeli za determinacijo (Sl. 2). Oba polža smo najprej določili na podlagi opisov zunanjih morfoloških znakov, opisanih v European non-marine molluscs, a guide for species identification (Welter-Schultes 2012), nato pa še na podlagi DNK-sekvenc podenote 1 citokrom C oksidaze. DNK smo uspešno izolirali iz vzorca tkiva iz konice repa in ga pomnožili z oligonukleotidnimi začetniki LCO1490 in HCO2198. Zaporedje so sekvencirali v Macrogen Europe (Amsterdam, Nizozemska) z uporabo istih začetnikov. Na podlagi dobljenih sekvenc in sekvenc morfološko podobnih vrst iz rodu slinarjev Limax in predstavnikov družine slinarjev Limacidae, ki živijo v Sloveniji, smo sestavili filogenetsko drevo (Sl. 6). Nekatere vrste v drevesu manjkajo, saj njihovih sekvenc v bazi GenBank ni. Novo število potrjenih vrst golih polžev v Sloveniji je tako 29. Acknowledgements We would like to thank Cene Fišer for helping us with slug determination and guiding us throughout the writing process, Rajko Slapnik for providing the information of the first find of Limax dacampi by Borut Kumar, and Borut Kumar for trusting us to publish his data. We also thank Marjan Vaupotič for his advice on studying slugs, Maja Zagmajster for providing the map of distribution, and the ARIS programme P1-0184 for funding this research. Special thanks go to everyone who helped with the fieldwork: to Romana Božič for helping search for Limax conemenosi, Blaž Pavlič, Lana Klemenčič, Nik Milek, Nik Šabeder and Val Milek with whom we searched for amphibians but discovered Limax dacampi, and to David Tomšič for his help during the second search for Limax dacampi. References Dhora D. 2014. Molluscs of Albania 2014: List of species and biogeographical data. Buletin Shkencor – Seria e shkencave të natyrës. 64: 149-181. 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The table is available as an online supplement at https://doi.org/10.14720/ns.26.2.21-33. © 2024 Maj Kastelic, Behare Rexhepi To je prostodostopen članek, objavljen pod določili licence Creative Commons Priznanje avtorstva 4.0 Mednarodna, ki dovoljuje neomejeno rabo, razširjanje in kopiranje v kakršnemkoli mediju ter obliki, pod pogojem, da sta navedena avtor in vir. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.