Chen, Zhi-Teng and Yu-Zhou Du. 2017. Supplementary illustrations for two Nemoura (Plecoptera: Nemouridae) species. Illiesia, 13(10):98-103. https://doi.org/10.25031/2017/13.10 http://zoobank.org/ urn:lsid:zoobank.org:pub:00287636-CF1A-4E80-947C-DC749079DE81 Illiesia – http://illiesia.speciesfile.org Volume 13 – Number 10 – Page 98 SUPPLEMENTARY ILLUSTRATIONS FOR TWO NEMOURA (PLECOPTERA: NEMOURIDAE) SPECIES Zhi-Teng Chen1and Yu-Zhou Du1, 2, 3 1 School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China. 2 Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education, Yangzhou University, Yangzhou 25009, China. 3 Corresponding author. E-mail: 741208116@qq.com; yzdu@yzu.edu.cn ABSTRACT Supplementary images for two Chinese Nemoura (Plecoptera: Nemouridae) species, N. fusiformis Chen & Du, 2017 and N. nankinensis Wu, 1926 are provided to allow for ease of future identifications. Corrected coordinates for the holotype and paratype locality are provided for N. fusiformis. Keywords: Plecoptera, Nemouridae, Nemoura, supplementary illustrations, China INTRODUCTION Chen & Du (2017) described a new species of Nemoura, N. fusiformis Chen & Du, 2017 from Jiangsu Province of east-central coastal China. These authors additionally illustrated N. nankinensis Wu, 1926, also a species described from Jiangsu Province of China. However, detailed structures of the male epiproct were not as clearly imaged because of older camera equipment. In this paper, supplementary images of the epiproct of both these two Nemoura species are presented to allow for more accurate identifications. Subsequent plotting of coordinates of the holotype male and paratype specimen data for N. fusiformis uncovered a significant error. New coordinates have been provided. MATERIAL AND METHODS Specimens used in this study were collected by hand and preserved in 75% ethanol. Morphology was studied with a Leica MZAPO microscope. Color images were taken with a KEYENCE VHX- 5000 camera. Coordinates were determined using GoogleEarth. All specimens used in this study are deposited in the Insect Collection of Yangzhou University (ICYZU), Jiangsu Province, China. Chen, Zhi-Teng and Yu-Zhou Du. 2017. Supplementary illustrations for two Nemoura (Plecoptera: Nemouridae) species. Illiesia, 13(10):98-103. https://doi.org/10.25031/2017/13.10 Illiesia – http://illiesia.speciesfile.org Volume 13 – Number 10 – Page 99 1 2 Chen, Zhi-Teng and Yu-Zhou Du. 2017. Supplementary illustrations for two Nemoura (Plecoptera: Nemouridae) species. Illiesia, 13(10):98-103. https://doi.org/10.25031/2017/13.10 Illiesia – http://illiesia.speciesfile.org Volume 13 – Number 10 – Page 100 Figs. 1-3. Nemoura fusiformis Chen & Du. 1. Male terminalia, dorsal view; 2. Male terminalia, lateral view; 3. Male epiproct, ventral view. RESULTS Nemoura fusiformis Chen & Du, 2017 http://lsid.speciesfile.org/urn:lsid:Plecoptera.speciesfile.org: TaxonName:500685 (Figs. 1-3) Nemoura fusiformis Chen & Du, 2017. Zootaxa. 4254 (2): 294. Type locality: Jiangsu Province, Suzhou. Type material. Holotype: male, China: Jiangsu Province, Suzhou, Mt. Qionglongshan, 36m, 31.2629 N, 120.4318 E, 29 March, 2016, leg. Zhi- Teng Chen, Qing-Bo Huo (ICYZU: CNJSSZN1). Material examined. 4 males, same data as holotype, 6 males; China: Jiangsu Province, Mt. Qionglongshan, 36 m, 31.2629 N, 120.4318 E, 23 February, 2016, leg. Zhi-Teng Chen, Qing-Bo Huo. Remarks. The epiproct of N. fusiformis is elliptical in dorsal view. Its dorsal sclerite is broad with a pair of oblique, medially connected, darkly sclerotized lateral arms. The arms cover the epiproct laterally and a pair of C-shaped sclerites is present at the apex of epiproct (Figs. 1-2). The ventral sclerite is spinulose with a pair of symmetrical, J-shaped ventral ridges that converge 3 Chen, Zhi-Teng and Yu-Zhou Du. 2017. Supplementary illustrations for two Nemoura (Plecoptera: Nemouridae) species. Illiesia, 13(10):98-103. https://doi.org/10.25031/2017/13.10 Illiesia – http://illiesia.speciesfile.org Volume 13 – Number 10 – Page 101 Figs. 4, 5. Nemoura nankinensis Wu. 4. Male terminalia, dorsal view; 5. Male terminalia, lateral view. Chen, Zhi-Teng and Yu-Zhou Du. 2017. Supplementary illustrations for two Nemoura (Plecoptera: Nemouridae) species. Illiesia, 13(10):98-103. https://doi.org/10.25031/2017/13.10 Illiesia – http://illiesia.speciesfile.org Volume 13 – Number 10 – Page 102 medially from base to the blunt apex (Fig. 3). The lateral knob is weakly sclerotized and covered with spinules (Figs. 1-2). This species is presently known only from the east-central coastal Jiangsu Province of China, north of Shanghai to Suzhou. Nemoura nankinensis Wu, 1926 http://lsid.speciesfile.org/urn:lsid:Plecoptera.speciesfile.org: TaxonName:6358 (Figs. 4-5) Nemoura nankinensis Wu, 1926. J. Sci. Arts. 5:332. Type locality: Jiangsu Province, Nanking; Wu, 1938. Plecopt. Sin., 182; Illies, 1966. Das Tierreich, 82:208. Type material (lost). Holotype: male, China: Jiangsu Province, Nanking, Ning-Kuo Temple, 4 April, 1923, leg. Chenfu Wu. Material examined. 18 males and 10 females, China: Jiangsu Province, Nanjing, Mt. Zijinshan, 18-19 February, 2006, leg. Zhi-Jie Wang; 1 male and 1 female, same locality, 2 February, 2016, leg. Zhi- Teng Chen, Zhi-Jie Wang; 41 males and 21 females, same locality, 17 February, 2016, leg. Zhi-Teng Chen, Qin-Bo Huo, Qiu-Yue Huo; 19 males and 18 females, same locality, 3 March, 2016, leg. Zhi-Teng Chen, Qin-Bo Huo. Remarks. The epiproct of N. nankinensis is oval with the dorsal sclerite medially having a pair of separated, transverse lateral arms reaching the ventral sclerite, and the anterior of the arms with a pair of circular, apically bifurcated sclerites. The ventral sclerite is broad with a row of long spines. The lateral knob is well developed but weakly sclerotized (Figs. 4-5). The holotype and all paratypes of N. nankinensis are lost (Wu 1962). The original description and illustrations by Wu (1926, 1938) were typically incomplete. Yang et al. (2015) repeated the original description and used the illustrations of Wu (1926). These authors indicated that the male genitalia of N. nankinensis was similar with N. yunnanensis Wu, 1940 (see fig. 275 in Yang et al. 2015), but this morphological similarity is difficult to ascertain with their available illustrations. Based on the male epiproct and cercal characters, N. nankinensis appears more similar with N. oculata Wang & Du, 2006 (see Figs. 15-21 in Wang et al. 2006) described from Guizhou Province of southwestern China. However, comprehensive morphological studies of both males and females of the many species of the genus Nemoura described or reported from China (DeWalt et al. 2017) are necessary before specific relationships can be determined more accurately. ACKNOWLEDGMENTS Dr. Boris C. Kondratieff, Dr. R. Edward DeWalt and Dr. Bill P. Stark assisted in improving the manuscript. We thank the Testing Center of Yangzhou University for help with the images. This research was supported by the National Natural Science Foundation of China (No. 31572295; 31071958), and by the fund of Excellent Doctoral Dissertations of Yangzhou University. REFERENCES Chen, Z.T. & Y.Z. Du. 2017. A new species of Nemoura (Plecoptera: Nemouridae) from Jiangsu Province, China, with new illustrations for Nemoura nankinensis Wu. Zootaxa, 4254 (2):294-300. https://doi.org/10.11646/zootaxa.4254.2.10 DeWalt, R.E., M.D. Maehr, U. Neu-Becker & G. Stueber. 2016. Plecoptera Species File Online. Version 5.0/5.0. Accessed 26 October 2016. http://plecoptera.speciesfile.org/ Illies. J. 1966. Katalog der rezenten Plecoptera. Das Tierreich – Eine Zusammenstellung und Kennzeichnung der rezenten Tierformen. 82:1- 631. Wu, C.F. 1926. Two new species of stoneflies from Nanking. The China Journal of Science and Arts, 5:331-332. Wu, C.F. 1938. Plecopterorum sinensium: A monograph of stoneflies of China (Order Plecoptera). Yenching University, Beijing, 225 pp. Wu, C.F. 1940. First supplement to the stoneflies of China (Order Plecoptera). Peking Natural History Bulletin, 14(2):153-157. Wu, C.F. 1962. Results of the Zoologico-Botanical expedition to Southwest China, 1955–1957 (Plecoptera). Acta Entomologica Sinica, 11(Supplement):139-153. Wang, Z.J., Y.Z. Du, I. Sivec, & Z.Z. Li. 2006. Records and descriptions of some Nemouridae Chen, Zhi-Teng and Yu-Zhou Du. 2017. Supplementary illustrations for two Nemoura (Plecoptera: Nemouridae) species. Illiesia, 13(10):98-103. https://doi.org/10.25031/2017/13.10 Illiesia – http://illiesia.speciesfile.org Volume 13 – Number 10 – Page 103 species (Order: Plecoptera) from Leigong Mountain, Guizhou province, China. Illiesia, 2(7):50-56. http://illiesia.speciesfile.org/papers/Illiesia02- 07.pdf Yang, D., W.H. Li & F. Zhu. 2015. Fauna Sinica, Insecta. Vol. 58. Plecoptera: Nemouroidea. Science Press, Beijing, 518 pp. Submitted 2 November 2017, Accepted 13 November 2017, Published 21 November 2017 Hosted and published at the University of Illinois, Illinois Natural History Survey, Champaign, Illinois, U.S.A.