34 KB490 KB31 KB200820252011Elmadfa, IbrahimFabian, ElisabethKósa, LajosPölöskey, PeterRéthy, Lajos Attilaštevilka:2letnik:4str. 24-33ISSN:1855-5640COBISSID:512159032URN:URN:NBN:SI:doc-ZQMDYHBBenMedicinska fakultetaActa medico-biotechnicaasthmaastmanitrosative stressoksidativni stresoxidative stresss Th17 povezani interlevkinistres, povezan z dušikovim oksidomTh17-related interleukinsInterlevkini, povezani s Th17, in parametri, povezanimi z dušikovim oksidom pri astmi otrok| Th17-related interleukins and parameters of oxidative/nitrosative events in childhood asthma|Purpose: Allergic asthma is a chronic lung disease with a major inflammatory component including reactive oxygen and nitrogen species in the background. The objective of this study was to investigate the relationship between the Th17-associated interleukins IL-6 and IL-10, and oxidative/nitrosative markers of stress in childhood atopic asthma. Methods: For this observational study 35 asthmatic (AG) and 21 healthy (CG) children were recruited; the volume of fractionated exhaled nitric oxide (Fe no) was measured with the NIOX nitric oxide monitoring system. Plasma levels of IL-6 and IL-10 were analysed with ELISA, malondialdehyde (MDA) with HPLC. Results: Compared to healthy controls, asthmatics exhaled a significantly (p<0.001) higher mean volume of Fe no and had significantly (p=0.012) enhanced levels of IL-6. A significant correlation between the Th17 activator IL-6 and Fe no could be found in the asthma (p=0.005) but not in the control group. The plasma concentration of IL-10 was significantly (p=0.002) lower in asthmatics than in controls and correlated significantly negatively with Fe no in both groups (AG:p=0.020; CG:p=0.008). Plasma levels of MDA, a lipid peroxidation product and indicator of oxidative stress, were significantly (p<0.001) enhanced in asthmatics. There was a significant (AG:p=0.001; CG:p=0.005) correlation between MDA and IL-6 but not of IL-10 in both groups. Conclusion: This study indicates a significant relationship between the Th17-related cytokines IL-6 and IL-10, pulmonary inflammation, and systemic oxidative stress in asthmatic children. The mechanisms involved in this process have not been fully elucidated an altered redox balance may, however, have considerable influence on asthmatic immunopathologyNamen: Alergična astma je kronična pljučna bolezen s pomembno vnetno komponento, vključno z reaktivnimi spojinami kisika in dušika. Namen raziskave je bil raziskati odnos s Th17 povezanimi interlevkini (IL-6 in IL-10) in markerji vnetja, povezanimi z dušikovim oksidom pri otroški atopični astmi. Metode: V raziskavo je bilo zajetih 35 otrok z astmo (AG) in 21 zdravih otrok (CG); z NIOX monitornim sistemom za dušikov oksid smo izmerili volumen frakcioniranega izdihanega dušikovega oksida (FENO). Nivo IL-6 in IL-10 v plazmi je bil analiziran z ELISA, malondialdehid (MDA) s HPLC. Rezultati: V primerjavi s kontrolno skupino so astmatiki izdihali pomembno večjo količino FENO (p < 0,001) in imeli pomembno višje vrednosti IL-6 (p = 0,012). Pomembna korelacija je bila med aktivatorjem Th17, ki ga predstavlja IL-6, in FENO pri astmatikih, ne pa tudi pri kontrolni skupini. Plazemska koncentracija IL-10 je bila pomembno nižja pri astmatikih (p = 0,002) v primerjavi s kontrolno skupino. V obeh skupinah smo ugotovili statistično pomembno negativno korelacijo med IL-10 in FENO (AG: p = 0,020; CG: p = 0,008). Plazemske vrednosti MDA, ki je lipidni produkt peroksidacije in indikator oksidativnega stresa, so bile pri astmatikih pomembno zvišane (p < 0,001). Pri obeh skupinah je bila pomembna korelacija med MDA in IL-6, ne pa zIL-10. Zaključek: Raziskava je potrdila pomembno povezanost s Th17 povezanimi citokini IL-6 in IL-10, vnetjem pljuč in sistemskim oksidativnim stresom pri otrocih z astmo. Mehanizmi, ki so vpleteni v te procese, še niso popolnoma razjasnjeni. Spremenjeno redoks ravnovesje ima najverjetneje pomemben vpliv na imunopatologijo astmeTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Mariboru, Medicinska fakulteta