28 KB113 KB25 KB199820252003Grdadolnik, Jože12 straništevilka:4letnik:50str. 777-788ISSN:1318-0207COBISSID:2956314URN:URN:NBN:SI:doc-WGFO4ERBenSlovensko kemijsko društvoActa chimica slovenicaFTIR spektroskopijaproteinispektriSaturation effects in FTIR spectroscopy| intensity of amide I and amide II bands in protein spectra|In the present article the dependence between the film thickness of the BSA protein and the intensity and structure of the Amide I and II bands are presented. Protein films thicker than 4 mum are shown to be unsuitable for structural investigation using transmission infrared spectroscopy since the signal for Amide I is saturated. Saturation of the Amide I band in the proteinfilm spectrum causes complete disruption in the structure of the Amide I band. Furthermore, the structure of the nearby Amide II band is also perturbed. Hence, in order to avoid such band distortions the protein film thickness should be below 4 mum, which corresponds to the absorption value of the strongest band in the spectrum below 1.2 absorption unitsTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko kemijsko društvo