26 KB1897 KB25 KB197320132004Čufar, KatarinaGričar, JožicaOven, PrimožZupančič, Martin12 straništevilka:75str. 135-146ISSN:0351-3114COBISSID:1438118URN:URN:NBN:SI:doc-WGBOPXLLslInštitut za gozdno in lesno gospodarstvoUniverza v Ljubljani, Biotehniška fakulteta, Oddelek za gozdarstvo & Oddelek za lesarstvoZbornik gozdarstva in lesarstvacelična diferenciacijakambijnavadna smrekaPicea abiessvetlobna mikroskopijavpliv temperatureOdziv kambija navadne smreke (Picea abies) na ogrevanje in hlajenje debla|The effect of experimental heating and cooling in a part of the stem of Norwayspruce (Picea abies) on cambial activity and cell differentiation was studied. Experiments were performed for 30 days; heating from 29 March 2004 until 3 May 2004 and cooling from 14 June, 2004 until 20 July 2004. Samples ofphloem, cambium and xylem were taken from trees every 10 days, transverse sections of tissue were prepared and observed under light microscope. After 10days, localized heating reactivated cambial cell division on the phloem sideand after 20 days on the xylem side as well. After 30 days up to 15 early wood cells were formed in the heat-treated sample. Regular cambial activity inthe control tree only started after 30 days. Cambial response to the coolingof the stem was less conspicuous. No anatomical differences were detected between the control and cool-treated samples after 10 and 20 days. After 30 days, late wood already started to form in the cool-treated sample and only transition early-late wood in the control tree. Our experiments demonstrated that it is possible to influence xylo- and phloemogenesis in Norway spruce by experimental heating and cooling of the stemProučili smo vpliv eksperimentalnega ogrevanja in hlajenja dela debla navadne smreke (Picea abies) na kambijevo aktivnost in celično diferenciacijo. Poskusasta potekala 30 dni; ogrevanje od 29.3.2004 do 3.5.2004, hlajenje pa od14.6.2004 do 20.7.2004. Vzorce floema, kambija in ksilema smo iz dreves odvzeli vsakih 10 dni, pripravili prečne prereze tkiv in preparate opazovali ssvetlobnim mikroskopom. Lokalno ogrevanje je po 10 dneh induciralo delitveno aktivnost kambija na floemsko stran, po 20 dneh pa tudi na ksilemsko. Po 30 dneh je pri ogrevanem vzorcu nastalo do 15 celic ranega lesa. V tem času se jeredna delitvena aktivnost kambija pri kontrolnem drevesu šele začela. Odziv kambija na hlajenje debla je bil manj izrazit. Anatomskih razlik med kontrolnim in hlajenim vzorcem po 10 in 20 dneh ni bilo. Po 30 dneh je pri hlajenem vzorcu začel nastajati kasni les, pri kontrolnem drevesu pa šele prehodni rani-kasni les. S poskusom smo demonstrirali, da je mogoče pri smrekiz umetnim ogrevanjem in hlajenjem debla vplivati na ksilo- in floemogenezoTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Ljubljani, Biotehniška fakulteta