800 KB28 KB200220262009Belušič, GregorMeglič, AndrejZupančič, Gregorštevilka:1letnik:52str. 19-28ISSN:1408-3671COBISSID:26360537URN:URN:NBN:SI:doc-WBOK6YQQenDruštvo biologov SlovenijeActa biologica slovenicaAnoksijaAnoxiaDipteraDvokrilcifiziologijakisikKisik, porabameritveMicroelectrodesMikroelektrodeMrežnicamuheOxygen ConsumptionRetinaUsing carbon fibre microelectrodes to monitor the oxidative metabolism of blowfly eyes| Uporaba mikroelektrod iz ogljikovih vlaken za spremljanje oksidativnega metabolizma mušjih oči|The oxidative metabolism in animal tissues can be conveniently monitored by measuring tissue PO2 with a carbon fibre microelectrode. We have established arecording configuration in a living animal by insertion of a carbon fibre electrode (CFE) into the retina of a blowfly (Calliphora vicina - chalky). Thecurrent flowing over an exposed carbon disc at the tip of an insulated carbon fibre with 5 micro m diameter is linearly proportional to PO2 when the PO2 was varied between 0 kPa (100% N2) and 100 kPa (100% O2) in the recording chamber. The slight changes in sensitivity of CFE during the recording time were corrected by calibrations performed at the start and at the end of the experiments. Exposure of the eye to bright light caused a drop in tissue P02. Hypoxia increased with the stimulation time, reaching a maximum after about 20s (deltaPO2=11.6 kPa). These results are in good agreement with direct measurements of O2 consumption in isolated eyesTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo biologov Slovenije