{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-RCYEJDL0/e1246a93-116c-4ef4-b19c-784bfbfd512c/HTML","dcterms:extent":"17 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-RCYEJDL0/ba741aaf-1fe8-4858-bc65-a49f5e83c52b/PDF","dcterms:extent":"948 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-RCYEJDL0/d8b6196c-7054-4e60-bbc8-5f10f1f30898/TEXT","dcterms:extent":"16 KB"}],"edm:TimeSpan":{"@rdf:about":"1992-2025","edm:begin":{"@xml:lang":"en","#text":"1992"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-RCYEJDL0","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:SPR-WQVGMCSC"},{"@xml:lang":"sl","#text":"Acta dermatovenerologica Alpina, Pannonica et Adriatica"}],"dcterms:issued":"2011","dc:creator":["Bogovac, Željka","Jančar, Nina","Kocjan, Boštjan","Lunar, Maja M.","Poljak, Mario","Seme, Katja"],"dc:format":[{"@xml:lang":"sl","#text":"letnik:20"},{"@xml:lang":"sl","#text":"številka:3"},{"@xml:lang":"sl","#text":"str. 135-139"}],"dc:identifier":["ISSN:1318-4458","COBISSID:29396697","URN:URN:NBN:SI:doc-RCYEJDL0"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Slovene Welding Society"},"dc:subject":[{"@xml:lang":"en","#text":"Cervical Intraepithelial Neoplasia"},{"@xml:lang":"en","#text":"Cervix Neoplasms"},{"@xml:lang":"en","#text":"Genetics"},{"@xml:lang":"sl","#text":"genetika"},{"@xml:lang":"sl","#text":"Genom virusni"},{"@xml:lang":"en","#text":"Genome, Viral"},{"@xml:lang":"sl","#text":"ginekologija"},{"@xml:lang":"sl","#text":"infekcije"},{"@xml:lang":"sl","#text":"Maternica, vrat, intraepitelijska neoplazija"},{"@xml:lang":"sl","#text":"maternični vrat"},{"@xml:lang":"sl","#text":"Maternični vrat, novotvorbe"},{"@xml:lang":"en","#text":"Papilloma"},{"@xml:lang":"en","#text":"Papillomavirus, Human"},{"@xml:lang":"en","#text":"Papilom"},{"@xml:lang":"sl","#text":"papiloma virusi"},{"@xml:lang":"sl","#text":"Papilomavirus človeški"},{"@xml:lang":"sl","#text":"Polimerazna, verižna reakcija"},{"@xml:lang":"en","#text":"Polymerase Chain Reaction"}],"dcterms:temporal":{"@rdf:resource":"1992-2025"},"dc:title":{"@xml:lang":"sl","#text":"Prevalence of HPV-16 genomic variant carrying a 63-bp duplicated sequence within the E1 gene in Slovenian women|"},"dc:description":{"@xml:lang":"sl","#text":"High-risk HPV, particularly HPV-16, is etiologically associated with the development of cervical cancer and its precursor lesions - cervical intraepithelial neoplasia (CIN). However, most precancerous lesions will not progress to cancer. Numerous studies have shown that HPV-16 consists of several genomic variants, which differ in their association with cervical cancer, viral persistence and the frequency of recurrence of cervical disease.Recently, a novel, presumably less pathogenic, HPV-16 E6-T350G genomicvariant has been identified, carrying a 63-bp in-frame insertion in theE1 gene. No data from Slovenian patients have so far been reported for thisspecific HPV-16 variant. In the present study, therefore, a total of 390 HPV-16 positive samples obtained from the same number of women with normal cytology, CIN I, CIN II, CIN III or cervical cancer, were analyzed. The HPV-16E1 insert variant was detected using real-time PCR-amplification of a 146-210-bp fragment of the E1 gene and PCR-sequencing of a 169-bp fragment of the E6 gene. The HPV-16 E1 insert variant was identified in 7/48 (14.6%), 1/21(4.8%), 2/20 (10.0%), 9/131 (6.9%) and 12/170 (7.1%) of women with normal cytology, CIN I, CIN II, CIN III and cervical cancer, respectively. All HPV-16E1 insert variants with an amplifiable E6 gene belonged to the European HPV-16 E6-350G variant group. No statistically significant differences in the prevalence of HPV-16 E1 insert genomic variant in women presenting with normalcytology and those with the different stages of HPV-16-induced disease were found"},"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-RCYEJDL0","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-RCYEJDL0"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-RCYEJDL0/ba741aaf-1fe8-4858-bc65-a49f5e83c52b/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Združenje slovenskih dermatovenerologov"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-RCYEJDL0/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-RCYEJDL0"}}}}