34 KB154 KB32 KB199820252003Falnoga, IngridMazej, DarjaStibilj, Vekoslava14 straništevilka:2letnik:50str. 185-198ISSN:1318-0207COBISSID:17565735URN:URN:NBN:SI:doc-R5QCJ2JEenSlovensko kemijsko društvoActa chimica slovenicakemijska analizakriselenspektrometrijaSelenium determination in whole blood, plasma and selenoprotein P by hydride generation atomic fluorescence spectrometry| Določanje selena v krvi, plazmi in selenoproteinu P s hidridno tehniko avtomatske fluorescenčne spektrometrije|Selenium was dtermined in whole blood, plasma and plasma protein fractions SeP and (GSH-Px+Alb) obtained by affinity chromatography. For the digestion ofwhole blood and plasma samples a simple digestion procedure using Hsub2SOsub4, Vsub2Osub2 and Hsub2SOsub4 solution was developed, while for the decomposition of plasma protein fractions HNOsub2 and Hsub2Osub2 were sufficient. After both procedures detection of Se was madeby HG-AFS and this combination gave reliable results. Dilution of the GSH-Px+Alb fraction was reduced by lyophilisation of pooled subfractions. The main advantages of the described procedures are low detection limit (0.2 ng Se/g of solution) and the large number of samples analysed in a short time as only two analyses are needed for determination of the percentage of Se in SeP in plasma sample. The procedures developed were used to determine selenium in real samples from which an average mass fraction of selenium in whole blood ofSlovenia ressidents of 87+ - 13 ng/g (n=43) and Se in SeP of 70 +- 9%(n=5) were found. Both values are comparable with letrature data for the general populationTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko kemijsko društvo