688 KB22 KB200420252016Černoša, IvaPirc, ŽanRenčelj, Andrejštevilka:1letnik:108str. 17-23ISSN:1581-9175ISSN:1854-1941COBISSID:88682753URN:URN:NBN:SI:doc-LSCKXE4FslBiotehniška fakultetaActa agriculturae Slovenicabody mass gaincandidate genescattlegeneticsgenetikagovedokandidatni genileptinprirast telesne maseVpliv SNP znotraj FTO in LEP gena na prirast telesne mase pri govedu|Body mass gain in a certain time period is one of the most important parameters in beef production. Some candidate genes, which have major impact on growth traits, are known from the literature, notably among them the FTO (fat mass and obesity-associated) and LEP (leptin) locus. In our experiment, we assessed the frequency of alleles at two polymorphic sites within the FTO gene (FTO6 and FTO10) and at polymorphic site in the LEP gene in a sample of Aberdeen Angus (AA) and Holstein Friesian (HF) breeds. In a larger sample, containing dairy and beef cattle breeds we tested the association of these three SNPs with the body mass gain in a 200-day period (between 165th and 365th day of age). In our material we could not confirm the linkage between two SNPs within the FTO locus, due to the existence of the region with high recombination rate between exon 5 and intron 5 in the FTO gene. We demonstrated significant impact of birth weight, breed and SNPs in the FTO gene on the growth. The effect of the polymorphic site within the LEP gene could not be confirmedPrirast telesne mase v določenem časovnem obdobju je eden pomembnejših parametrov v prireji govejega mesa. Znanih je nekaj kandidatnih genov, ki imajo velik vpliv na lastnosti rastnosti, med njimi zavzemata pomembno mesto lokusa FTO (povezan z maso maščevja in debelostjo) in LEP (leptin). V poskusu smo ocenili frekvence alelov na dveh lokusih znotraj gena FTO (FTO6 in FTO10) in na enem lokusu znotraj leptinskega gena na vzorcu goveda pasme aberdeen angus (AA) in črno%bele (ČB) pasme. Povezavo teh lokusov s prirasti v 200 dnevnem obdobju (med 165. in 365. dnem starosti) smo preverjali na vzorcu mešane populacije mlečnih in mesnih pasem. Vezave med obema polimorfizmoma znotraj FTO gena nismo mogli potrditi, ker se med eksonom 5 in intronom 5 v FTO genu nahaja mesto z visoko frekvenco rekombinacij. Na preiskovani populaciji smo dokazali značilen vpliv telesne mase ob rojstvu, pasme in polimorfizmov v FTO genu na prirast. Vpliva polimorfizma v genu LEP nismo mogli potrditiTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Ljubljani, Biotehniška fakulteta