{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-K70TEAQG/3d4a7257-656a-4dd6-828e-ead1b54125ed/HTML","dcterms:extent":"44 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-K70TEAQG/e340a7ba-13b7-45f3-9e7b-32f34d32de2a/PDF","dcterms:extent":"202 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-K70TEAQG/20091d36-3a9e-4c40-919e-9a63311be6fc/TEXT","dcterms:extent":"25 KB"}],"edm:TimeSpan":{"@rdf:about":"1992-2025","edm:begin":{"@xml:lang":"en","#text":"1992"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-K70TEAQG","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-FNIFVE9S"},{"@xml:lang":"sl","#text":"Radiology and oncology (Ljubljana)"}],"dcterms:issued":"2004","dc:creator":["Eynard, Natalie","Ganeva, Valentina","Rols, Marie-Pierre","Teissie, Justin","Vernhes, Marie Christine"],"dc:format":[{"@xml:lang":"sl","#text":"številka:2"},{"@xml:lang":"sl","#text":"letnik:38"},{"@xml:lang":"sl","#text":"9 strani"},{"@xml:lang":"sl","#text":"str. 121-129"}],"dc:identifier":["ISSN:1318-2099","COBISSID:18167001","URN:URN:NBN:SI:doc-K70TEAQG"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Association of Radiology and Oncology"},"dc:subject":[{"@xml:lang":"sl","#text":"celična membrana"},{"@xml:lang":"sl","#text":"Celična membrana, propustnost"},{"@xml:lang":"en","#text":"Cell Membrane Permeability"},{"@xml:lang":"sl","#text":"CHO celice"},{"@xml:lang":"en","#text":"Cho Cells"},{"@xml:lang":"sl","#text":"citologija"},{"@xml:lang":"en","#text":"Electroporation"},{"@xml:lang":"sl","#text":"Elektroporacija"},{"@xml:lang":"sl","#text":"permeabilnost celične membrane"}],"dcterms:temporal":{"@rdf:resource":"1992-2025"},"dc:title":{"@xml:lang":"sl","#text":"Ex vivo flow mammalian cell electropulsation|"},"dc:description":[{"@xml:lang":"sl","#text":"Cell electropulsation brings a local and reversible permeabilization of cell membranes. This gives the technical possibility to introduce (load) exogenous compounds (drugs, proteins, DNA) into cells. Flow-through electropulsation allows to treat a large volume (number) of cells as requested for cell therapy. Cells are flowing through a pulsing chamber where they are submitted to a well-defined number of calibrated pulses. A proper setting of pulse frequency and flow rate controls the number of pulses. A large volume of cellscan therefore be electrotreated in a small sized pulsing chamber. The viability of pulsed cells appears to be greatly preserved"},{"@xml:lang":"sl","#text":"Elektroporacija celic povzroči lokalno in reverzibilno permeabilizacijo celične membrane. To omogoča vnašanje eksogenih snovi v celice, kot so zdravila proteini in DNA. Pretočna elektroporacija omogoča tretman velike količine celic, kar je potrebno v primeru celične tarpije. Celice tečejo skozikomoro za elektroporacijo, kjer so izpostavljene točno dolečenim pogojem elektroporacije. Potrebno je prilagoditi frekvenco električnih pulzov, hitrostpretoka celic in število dovedenih pulzov. Na ta način je mogoče z elektroporacijo vnesti v celice velik volumen celic v majhni komori za elektroporacijo. Tudi viabilnost celic po elektroporaciji je zelo dobro ohranjena"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-K70TEAQG","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-K70TEAQG"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-K70TEAQG/e340a7ba-13b7-45f3-9e7b-32f34d32de2a/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Društvo radiologije in onkologije"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-K70TEAQG/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-K70TEAQG"}}}}