1277 KB17 KB200220262005Jezernik, KristijanVeranič, PeterZlatolas, Katarinaštevilka:1letnik:48str. 13-20ISSN:1408-3671COBISSID:20710361URN:URN:NBN:SI:doc-IUJKLBUWenDruštvo biologov SlovenijeActa biologica slovenicaceliceCelične kultureCelično gibanjeCell MovementCells, Culturedcitologijafotografijagibanje celicMicroscopy, VideoMikroskopija, videoUrotelijUrotheliumAnalysis of cell motility with a successive sequence of images| Analiza gibanja celic s serijo zaporednih fotografij|Abstract. Analysis of cell motility usually requires several hours of continuous video recording of a single view field. Consequently, the method isvery much time consuming and only a small number of cells is usually included into analysis. To increase the number of cells monitored in an experiment we developed a simple and expeditious method for analysing the cellmotility. A combination of successive series of images of individual cellsrelocated on CELLocate coverslips and vector graphic editing software Adobe Illustrator 9.0 enabled simultaneous monitoring of locomotion for up to ten times more cells as with previous methodsIzvleček. Analiza celičnega gibanja ponavadi zahteva večurno snemanje enega vidnega polja z video kamero. Metoda je zato zamudna in le malo celic se lahkovključi v analizo. Z namenom povečanja števila celic v določenem poizkusu, smo razvili preprosto in hitro metodo za analizo celičnega gibanja. S kombinacijo zaporednega slikanja istega vidnega polja, kar nam je omogočila uporaba CELLokate krovnih stekelc, in vektorskega grafičnega programa Adobe Illustrator 9.0, smo lahko v analizo gibanja celic vključili do desetkrat več celic, v primerjavi z dosedanjimi metodamiTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo biologov Slovenije