17 KB96 KB14 KB199220252006Baran, EugeniuszBaran, WojciechMazur, GrzegorzSzepietowski, Jacek C.letnik:15številka:3str. 113-118ISSN:1318-4458COBISSID:21825753URN:URN:NBN:SI:doc-FRXJ2EWFenSlovene Welding SocietyActa dermatovenerologica Alpina, Pannonica et AdriaticaAleliAllelesGeneticsgenetikaGenotipGenotypekožne bolezniPolimerazna, verižna reakcijaPolimorfizem (genetika)Polymerase Chain ReactionPolymorphism (Genetics)Promoter Regions (Genetics)Promoterska področja (genetika)PsoriasisPsoriazaTumor Necrosis FactorTumor nekrotizirajoči faktorA -308 promoter polymorphism of tumor necrosis factor alpha gene does not associate with the susceptibility to psoriasis vulgaris. No difference either between psoriasis type I and type II patients|Background: Genes encoded within the MHC complex on chromosome 6 are thought to play an important role in the pathogenesis of psoriasis. A potential candidate is tumor necrosis factor alpha (TNF-alpha) gene. Psoriasis features an increased activity of the proinflammatory cytokine TNF-alpha in affected lesions. TNF-alpha promoter region contains several polymorphisms, including GžA transition at position -308, which influences transcriptional activity of TNF-alpha. This study was performed to investigate the association between TNF-alpha single nucleotide polymorphism and susceptibility for psoriasis vulgaris. Material and methods: DNA from 78 patients with psoriasis vulgaris and 74 healthy volunteers with no personal and family history of psoriasis wasinvestigated. TNF-alpha promoter gene single nucleotide polymorphism (SNP) in position - 308 was evaluated by PCR-SSP The results were compared between group of psoriatic patients, divided into early onset of psoriasis (type I) and late onset of psoriasis (type II) subgroups, and healthy subjects. Results: There were no significant differences in the polymorphism of TNF-alpha promoter -308 (genotype distribution, allele frequencies) between psoriasis patients and healthy controls. Similar results were obtained analyzing subgroups of psoriasis patients (type I and type II of psoriasis) and gender groups. Conclusion: TNF-alfa promoter single nucleotide polymorphism (-308) is not associated with susceptiy bility to psoriasis vulgarisTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaZdruženje slovenskih dermatovenerologov