{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-BZNH5KBG/fbc467fc-c193-40e0-a37e-768c6aa9aeba/HTML","dcterms:extent":"28 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-BZNH5KBG/c5781b74-95b6-4a19-a115-c698f74b28c7/PDF","dcterms:extent":"148 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-BZNH5KBG/16d51265-c8ee-4c1f-bc3a-a7ba29f4e41f/TEXT","dcterms:extent":"23 KB"}],"edm:TimeSpan":{"@rdf:about":"1998-2025","edm:begin":{"@xml:lang":"en","#text":"1998"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-BZNH5KBG","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-KC6O72BG"},{"@xml:lang":"sl","#text":"Acta chimica slovenica"}],"dcterms:issued":"2009","dc:creator":["Bratkovič, Tomaž","Gaser, Dominik","Kreft, Samo","Lunder, Mojca","Pungerčar, Jože","Štrukelj, Borut"],"dc:format":[{"@xml:lang":"sl","#text":"številka:3"},{"@xml:lang":"sl","#text":"letnik:56"},{"@xml:lang":"sl","#text":"str. 712-717"}],"dc:identifier":["ISSN:1318-0207","COBISSID:2656113","URN:URN:NBN:SI:doc-BZNH5KBG"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"dc:subject":[{"@xml:lang":"sl","#text":"amoditoksin C"},{"@xml:lang":"sl","#text":"bakteriofagi"},{"@xml:lang":"sl","#text":"pankreatična fosfolipaza A2"},{"@xml:lang":"sl","#text":"peptidni ligandi"}],"dcterms:temporal":{"@rdf:resource":"1998-2025"},"dc:title":{"@xml:lang":"sl","#text":"Cross-affinity of peptide ligands selected from phage display library against pancreatic phospholipase A2 and ammodytoxin C|"},"dc:description":[{"@xml:lang":"sl","#text":"Two phage-displayed random peptide libraries were screened for ligands with potential inhibitory activity against pancreatic phospolipase A2 or ammodytoxin C (neurotoxin found in the venom glands of Vipera ammodytes ammodytes). The interaction of selected peptides with pancreatic phospholipase A2 and ammodytoxin C was confirmed with surface plasmon resonance and phage ELISA assays. Interestingly, peptides showed equal affinity to both proteins, regardless which of the two proteins was used as a target in the selection procedure. Despite pronounced affinity, none of the synthetic peptides inhibited enzyme targets in vitro at the concentrations below 167 microM"},{"@xml:lang":"sl","#text":"Pri iskanju ligandov s potencialno inhibitorno aktivnostjo na pankresno fosfolipazo A2 ali amoditoksin C (nevrotoksin iz strupnih žlez modrasa Vipera ammodytes ammodytes) smo uporabili dve knjižnici naključnih peptidov, izraženih na bakteriofagih. Peptidi, ki smo jih selekcionirali z različnimi selekcijskimi protokoli, jasno kažejo afiniteto do pankreasne fosfolipaze in amoditoksina C, ki smo jo določili s pomočjo površinske plazmonske resonance in encimskoimunskega testa. Peptidi presenetljivo izkazujejo podobno afiniteto do obeh proteinov, ne glede, katerega smo uporabili kot ciljno molekulo pri selekciji. Navkljub nedvoumni afiniteti, peptidi v območju testiranih koncentracij niso inhibirali fosfolipaznega delovanja in vitro"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-BZNH5KBG","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-BZNH5KBG"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-BZNH5KBG/c5781b74-95b6-4a19-a115-c698f74b28c7/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-BZNH5KBG/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-BZNH5KBG"}}}}