{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-BV7SZB6E/351fd2d8-2198-47c4-a8f0-4ee90af38bfd/PDF","dcterms:extent":"150 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-BV7SZB6E/d3f8dcec-e29e-41d2-bdc8-5aae7d2febe0/TEXT","dcterms:extent":"0 KB"}],"edm:TimeSpan":{"@rdf:about":"2004-2025","edm:begin":{"@xml:lang":"en","#text":"2004"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-BV7SZB6E","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-1fyqipbt"},{"@xml:lang":"sl","#text":"Acta agriculturae Slovenica"}],"dcterms:issued":"2007","dc:creator":["Avguštin, Gorazd","Gorenc, Gregor","Lukáš, Filip"],"dc:format":[{"@xml:lang":"sl","#text":"številka:2"},{"@xml:lang":"sl","#text":"letnik:90"},{"@xml:lang":"sl","#text":"str. 107-113"}],"dc:identifier":["ISSN:1581-9175","COBISSID:2216328","URN:URN:NBN:SI:doc-BV7SZB6E"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Univerza v Ljubljani, Biotehniška fakulteta"},"dc:subject":[{"@xml:lang":"sl","#text":"bakterije"},{"@xml:lang":"sl","#text":"mikrobi"},{"@xml:lang":"sl","#text":"mikrobiologija"},{"@xml:lang":"sl","#text":"prežvekovalci"},{"@xml:lang":"sl","#text":"vamp"}],"dcterms:temporal":{"@rdf:resource":"2004-2025"},"dc:title":{"@xml:lang":"sl","#text":"Examination of AI-2 quorum sensing system Prevotella bryantii and Prevotella ruminicola-like strain by using bioluminiscence assay| Ugotavljanje sistema za zaznavanje kritične celične gostote AI-2 pri bakteriji Prevotella bryantii in sevih, ki so podobni bakteriji Prevotella ruminicola, s testom bioluminiscence|"},"dc:description":[{"@xml:lang":"sl","#text":"Rumen is an example of a complex microbial ecosystem where extensive intraspecies as well as interspecies cell-cell cross-communication is expected, but little is known about. Four ruminal Prevotella strains, Prevotella bryantii B14, TC1-1 and TF1-3, and P. ruminicola-like strain 223/M2/7A were examined for the presence of AI-2 tzpe quorum sensing systems. Vibrio harveyi BB170 autoinducer bioassay was used in order to detect the production of AI-2 autoinducer in examined strains. Optimization of autoinducer bioassay was made to test the influence of sampling time and the substrate used in chemically defined medium CD on reporter strain luminescence signal. We discovered that AI-2 tzpe qurum sensing system is present in P. ruminicola-like strain 223/M2/7A, which induced more than 74% of reporter strain bioluminescence, regardless of the presence of glucose in chemically defined medium CD. Such induction of bioluminescence was not observed in P. bryantii strains, however"},{"@xml:lang":"sl","#text":"Vamp prevežkovalcev predstavlja kompleksen mikrobni ekosistem, kjer pričakujemo obsežne tako znotrajvrstne kot tudi medvrstne medcelične komunikacije, vendar je o njih le malo znanega. Pri štirih vampnih sevih iz rodu Prevotella, t.j. Prevotella bryantii B14, TC1-1 in TF1-3 ter P. ruminicola-podobnem sevu 223/M2/7A smo preverili prisotnost sistemov za zaznavanje celične gostote tipa AI-2. Z uporabo biološkega testiranja avtoinduktorjev s testnim organizmom Vibrio harveyi BB170 smo ugotavljali produkcijo molekul AI-2 pri preučevanih mikroorganizmih. S preverjanjem vpliva časa biološkega testiranja ter substrata, uporabljenega v kemijsko definiranem gojišču CD, na signal bioluminiscence reporterskega seva, smo optimizirali biološko testiranje avtoinduktorskih molekul. Ugotovili smo, da je AI-2 tip sistema za medcelično komunikacijo prisoten pri P. ruminicola podobnem sevu 223/M2/7A, ki je induciral več kot 74 % bioluminiscence reporterskega seva, ne glede na prisotnost glukoze v kemijsko definiranem gojišču CD. Indukcije bioluminiscence pri sevih vrste P. bryantii nismo opazili"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-BV7SZB6E","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-BV7SZB6E"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-BV7SZB6E/351fd2d8-2198-47c4-a8f0-4ee90af38bfd/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Univerza v Ljubljani, Biotehniška fakulteta"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-BV7SZB6E/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-BV7SZB6E"}}}}