{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-AX1NRQC3/65b39af6-aa18-4689-b24c-52b9728dc7e3/PDF","dcterms:extent":"1285 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-AX1NRQC3/50296236-e0b1-46fc-bd52-41e8dd2a2d1f/TEXT","dcterms:extent":"40 KB"}],"edm:TimeSpan":{"@rdf:about":"1992-2025","edm:begin":{"@xml:lang":"en","#text":"1992"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-AX1NRQC3","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:SPR-WQVGMCSC"},{"@xml:lang":"sl","#text":"Acta dermatovenerologica Alpina, Pannonica et Adriatica"}],"dcterms:issued":"2016","dc:creator":["Fujs Komloš, Kristina","Hošnjak, Lea","Kocjan, Boštjan","Poljak, Mario","Seme, Katja"],"dc:format":[{"@xml:lang":"sl","#text":"letnik:25"},{"@xml:lang":"sl","#text":"številka:iss. 4"},{"@xml:lang":"sl","#text":"str. 65-71"}],"dc:identifier":["ISSN:1318-4458","COBISSID:33016281","URN:URN:NBN:SI:doc-AX1NRQC3"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Slovene Welding Society"},"dc:subject":[{"@xml:lang":"en","#text":"detection"},{"@xml:lang":"sl","#text":"diferenciacija"},{"@xml:lang":"en","#text":"differentiation"},{"@xml:lang":"en","#text":"human papillomavirus types"},{"@xml:lang":"sl","#text":"humani papiloma virusi"},{"@xml:lang":"sl","#text":"odkrivanje"}],"dcterms:temporal":{"@rdf:resource":"1992-2025"},"dc:title":{"@xml:lang":"sl","#text":"Development of a novel multiplex type-specific quantitative realtime PCR for detection and differentiation of infections with human papillomavirus types HPV2, HPV27, and HPV57|"},"dc:description":{"@xml:lang":"sl","#text":"Introduction: The present study describes the development and evaluation of the first multiplex type-specific quantitative realtime PCR (RT-PCR), enabling simple, rapid, sensitive, and specific concurrent detection and differentiation of human papillomavirus (HPV) types HPV2, 27, and 57 in a single PCR reaction. Results: The HPV2/27/57 multiplex RT-PCR with a dynamic range of seven orders of magnitude (discriminating 10 to 10sup8 viral genome equivalents/reaction) has an analytical sensitivity of at least 10 viral copies of each targeted HPV type/reaction, and no cross-reactivities were observed among the included targets. All three primer/probe combinations were efficient in amplifying 500 copies of targeted DNA in a background of 10sup8, 10sup7, 500, 100, and 10 copies of non-targeted viral DNA/reaction, and the performance of the HPV2/27/57 multiplex RT-PCR was additionally not affected by the presence of background human genomic DNA. When testing DNA isolates obtained from fresh-frozen tissue specimens of various children's warts, the results of the HPV2/27/57 multiplex RT-PCR were completely in line with the results of the conventional Low-risk Alpha-PV PCR. Conclusion: The newly developed HPV2/27/57 multiplex RT-PCR is an appropriate test for use in routine clinical laboratory settings and for studies focusing on the molecular epidemiology, pathogenesis, and natural history of HPV2/27/57-related lesions"},"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-AX1NRQC3","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-AX1NRQC3"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-AX1NRQC3/65b39af6-aa18-4689-b24c-52b9728dc7e3/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Združenje slovenskih dermatovenerologov"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-AX1NRQC3/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-AX1NRQC3"}}}}