30138 KB65 KB199220252023Abecasis, Ana BLunar, Maja M.Mlakar, JanaPečavar, BlažPoljak, MarioPoljak, MarioTomažič, JanezTurel, GabrieleVandamme, Anne-MiekeVovko, Tomažletnik:32številka:iss. 3str. 99-110DOI:10.15570/actaapa.2023.20ISSN:1318-4458COBISSID_HOST:167503619URN:URN:NBN:SI:doc-9RCG33ZOenSlovene Welding SocietyActa dermatovenerologica Alpina, Pannonica et AdriaticaCentral EuropefilogenijaHIV-1molecular epidemiologymolekularna epidemiologijane-B podtipnon-B subtypephylogenypodtipiziranjesrednja EvropasubtypingHIV-1 subtype diversity and phylogenetic insight into non-B subtype transmission in Slovenia, 1989-2013|Introduction: Disease progression, drug resistance mutations, and treatment strategies may vary by HIV-1 subtype. This study determined HIV-1 subtypes circulating in Slovenia, a Central European country with an HIV-1 epidemic driven by men who have sex with men, focusing on molecular epidemiology of non-B subtypes. Methods: A total of 367 HIV-1 sequences were included. Subtype was assigned by employing eight different HIV subtyping tools coupled with maximum likelihood phylogenetic analyses. Results: The subtyping tools COMET, jpHMM, and REGA 3.0 exhibited the best performance on the dataset studied. Phylogenetic analyses showed a 14.7% prevalence of non-B subtypes, with subtype A detected most frequently (4.9%), followed by CRF02_AG (2.4%), subtype C (1.1%), subtypes D, G, and CRF01_AE (0.8% each), and subtypes F and CRF22_01A1 (0.3% each). A subtype could not be assigned to 12 sequences (3.3%), indicating potential unique recombinant forms. Non-B subtypes were significantly associated with a heterosexual route of transmission and infection acquired in Eastern Europe, Africa, or Asia. Conclusions: In a country where subtype B is predominant, non-B subtypes were observed in one out of seven patients, a nonnegligible proportion, which underlines the importance of systematic surveillance of HIV subtype diversity and the corresponding molecular epidemiologyTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaZdruženje slovenskih dermatovenerologov