195 KB0 KB200520252024Čerenak, AndrejaJakše, JernejRadišek, SebastjanVolk, Helenaštevilka:31str. 27-36ISSN:0350-0756COBISSID:221323779URN:URN:NBN:SI:doc-9EXRGGY4enInštitut za hmeljarstvo in pivovarstvoHmeljarski biltenCBCVdhmeljhopsqPCRviroidviroidsValidation of the potential of highly expressed transcripts as markers of early stages of cbcvd infection of hops|This study evaluated the expression of two candidate transcripts (1902 and 94443) in response to Citrus Bark Cracking Viroid (CBCVd) infection in three hop cultivars: Celeia, Styrian Cardinal, and Styrian Wolf. A root grafting technique was used for plant inoculation, and leaf samples were collected before symptoms appeared. The transcripts were selected based on bioinformatic analysis of RNA-seq data, that suggested high transcript levels in CBCVd-infected plants. qPCR results did not fully confirm this in the early stages of infection, the highest transcript expression was observed in CBCVd-infected Styrian Wolf at 4 months past inoculation, but surprisingly, expression dropped by 5 months past inoculation. This initial analysis offers insights into transcript expression during CBCVd infection, but further research is needed to confirm these findings and explore the potential of these transcripts as early infection markersTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaInštitut za hmeljarstvo in pivovarstvo Slovenije