236 KB40 KB200220242007Devjak, RokJuvan, PeterRežen, TadejaRozman, Damjanaletnik:12številka:2str. 1-13ISSN:1318-2129COBISSID:23707353URN:URN:NBN:SI:doc-971L8S8XslSlovensko društvo za medicinsko informatikoInformatica Medica SlovenicaAntilipemic AgentsbiokemijaCelične kultureCells, CulturedDrug EffectsGene Expressiongenomikagenske mikromrežeGensko izražanjeJetraklinična diagnostikaLipolitikiLiverOligonucleotide ProbesOligonukleotidne sondeRifampinKontrola kvalitete DNA mikromrež nizke gostote| Quality control of low-density DNA microarrays|DNA microarray technology, showing great perspective in clinical diagnostics and prognostics, is facing a problem of loosely defined and non-unified data quality control. We have developed a general approach for controlling the quality of low density DNA microarrays, which is based on the analysis of covariance of control probes. We have applied the approach to the Steroltalk microarray, which was developed to study genes in connection with cholesterol homeostasis. We analyzed changes in genes expression in human primary hepatocytes treated with rifampicin and rosuvastatin and evaluated the approach by comparing our results to these from the literature. The proposed approach is independent of the type of DNA microarray and therefore may contribute to more rapid transfer of this technology to the clinical practiceTehnologija DNA mikromrež, ki veliko obeta na področju klinične diagnostike inprognostike, se sooča s problemom slabo izoblikovane in neenotne sheme za kontrolo kvalitete meritev. Razvili smo splošen pristop za kontrolo kvalitete DNA mikromrež nizke gostote, ki je osnovan na analizi kovariance kontrolnih sond. Pristop smo aplicirali na mikromreži Steroltalk, razviti z namenom preučevanja izražanja genov, povezanih s homeostazo holesterola. Na modelu primarnih človeških hepatocitov smo analizirali spremembe v izražanju genov pri aplikaciji riiampicina in rosuvastatina in pristop ovrednotili s primerjavo rezultatov analize z navedbami iz literature. Predlagan pristop je neodvisen od vrste uporabljene DNA mikromreže in kot tak lahko pomembno prispeva k hitrejšemu prenosu te tehnologije v klinično praksoTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko društvo za medicinsko informatiko