{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-8HD3J269/5e8338cf-491e-4f3d-94e4-c2670d8ec2e2/HTML","dcterms:extent":"32 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-8HD3J269/ac9ee122-4cd5-4a85-9e7f-126090068008/PDF","dcterms:extent":"1157 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-8HD3J269/cb488228-e88d-47c9-bdff-068302b48d29/TEXT","dcterms:extent":"28 KB"}],"edm:TimeSpan":{"@rdf:about":"1998-2025","edm:begin":{"@xml:lang":"en","#text":"1998"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-8HD3J269","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-KC6O72BG"},{"@xml:lang":"sl","#text":"Acta chimica slovenica"}],"dcterms:issued":"2006","dc:creator":["Carl, Nejc","Drobnak, Igor","Lah, Jurij","Šumiga, Boštjan","Vesnaver, Gorazd"],"dc:format":[{"@xml:lang":"sl","#text":"številka:3"},{"@xml:lang":"sl","#text":"letnik:53"},{"@xml:lang":"sl","#text":"8 strani"},{"@xml:lang":"sl","#text":"str. 284-291"}],"dc:identifier":["ISSN:1318-0207","COBISSID:27964933","URN:URN:NBN:SI:doc-8HD3J269"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"dc:subject":[{"@xml:lang":"sl","#text":"biokemija"},{"@xml:lang":"sl","#text":"cirkularni dikroizem"},{"@xml:lang":"sl","#text":"distamicin"},{"@xml:lang":"en","#text":"DNA"},{"@xml:lang":"sl","#text":"fizikalna kemija"},{"@xml:lang":"sl","#text":"interakcije"},{"@xml:lang":"sl","#text":"izotermna titracijska kalorimetrija"},{"@xml:lang":"sl","#text":"karakterizacija"},{"@xml:lang":"sl","#text":"makromolekule"},{"@xml:lang":"sl","#text":"netropsin"},{"@xml:lang":"sl","#text":"termodinamika"}],"dcterms:temporal":{"@rdf:resource":"1998-2025"},"dc:title":{"@xml:lang":"sl","#text":"Competition of some minor groove binders for a single DNA binding site|"},"dc:description":[{"@xml:lang":"sl","#text":"We employed circular dichroism (CD) and isothermal titration calorimetry (ITC) to characterize binding of netropsin (NET) and distamycin A (DST) to the hairpin (D) formed from the 5-CGAATTGACGTCTCCGTCAATTCG-3 oligonucleotide. From fitting the appropriate model to the CD titration curves describing NET and DST binding to the D and CD and ITC titration curves describing the displacement of NET bound to D by the added DST and vice versa we were able to determine the corresponding binding and displacement constants at 25 °C. The displacement constant (K12) determined for 2DST + NET-D - DST2-D + NET process is in good agreement with the corresponding value calculated from the individual binding events (NET + D -NET-D, k1 = 1.6 106 M-1 DST + D -DST-D, k1 = 1.0 107 M-1 and DST + DST-D -DST2-D, k2 = 0.9 104 M-1). ITC results reveal that the free energy of displacement .G12° = -29 kJ mol-1 in combination with the enthalpy of displacement .H12° = -74 kJ mol-1 results in the corresponding entropy contribution T.S12° = -45 kJ mol-1. Evidently, the 2DST + NET-D -DST2-D + NET displacement is a strongly enthalpy driven process. What we find important with the displacement studies is the observation that NET entirely displaces DST from one half of its 1:1 complexes with the hairpin in spite of the fact that the 1:1 binding constant of NET is about one order of magnitude lower than the corresponding 1:1 constant of DST. We propose that the driving force of this displacement is strong binding of DST molecules that are displaced as a result of the overall NET-DST-D equilibrium to the available DST-hairpin 1:1 complexes"},{"@xml:lang":"sl","#text":"Za karakterizacijo vezave netropsina (NET) in distamicina A (DST) na lasnico (D), ki se tvori iz oligonukleotida 5-CGAATTGACGTCTCCGTCAATTCG-3 smo uporabili cirkularni dikroizem (CD) in izotermno titracijsko kalorimetrijo (ITC). S prilagajanjem primernih modelnih funkcij k CD titracijskim krivuljam, ki popisujejo vezavo NET in DST na lasnico D in ITC titracijskim krivuljam, ki popisujejo izpodrivanje na lasnico vezanega NET z dodanim DST in obratno, smo uspeli določiti ustrezne konstante vezanja in izpodrivanja pri 25 °C. Konstanta izpodrivanja K12 določena za proces 2DST + NET-D - DST2-D + NET se dobro ujema z vrednostjo K12 izračunano s pomočjo konstant vezanja doloeenih za ustrezna posamična vezanja (NET + D -NET-D, k1 = 1.6 106 M-1 DST+ D -DST-D, k1 = 1.0 107 M-1 and DST + DST-D -DST2-D, k2 = 0.9 104 M-1). ITC rezultati pokažejo, da vodi prosta entalpija izpodrivanja G12° = -29 kJ mol-1 v kombinaciji z entalpijo izpodrivanja H12° = -74 kJ mol-1 do ustreznega entropijskega prispevka TS12° = -45 kJ mol-1, kar pomeni, da je izpodrivanje 2DST + NET-D -DST2-D + NET močno entalpijsko voden proces. Rezultat tega dela, ki se nam zdi se posebej pomemben je ugotovitev, da NET popolnoma izpodrine DST iz ene polovice njegovih 1 : 1 kompleksov z lasnico kljub temu, da je 1 : 1 konstanta vezanja za NET približno za en velikostni red nizja od ustrezne 1 : 1 konstante vezanja za DST. Razlaga, ki jo predlagamo je, da je gonilna sila omenjenega izpodrivanja močno vezanje prostih molekul DST določenih s celotnim NET-DST-D ravnotezjem na DST:D = 1 : 1 komplekse prisotne v raztopini"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-8HD3J269","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-8HD3J269"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-8HD3J269/ac9ee122-4cd5-4a85-9e7f-126090068008/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-8HD3J269/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-8HD3J269"}}}}