2759 KB17 KB199220252001Ružić-Sabljić, Evaletnik:10številka:4str. 143-147ISSN:1318-4458COBISSID:14523353URN:URN:NBN:SI:doc-61MTDD2QenSlovene Welding SocietyActa dermatovenerologica Alpina, Pannonica et AdriaticaAntibodies, bacterialBorrelia burgdorferidiagnostikaIsolation and purificationLyme boreliozaLyme diseaseLymska bolezen sin Lymska boreliozaMicrobiologyPolimeraza, verižna reakcijaPolymerase chain reactionProtitelesa bakterijskaMicrobiological diagnosis of Lyme borreliosis|Lyme borreliosis is a complex multisystem disorder. Clinical manifestations are variable and differential diagnosis is often difficult. Besides clinical criteria (erythema migrans), the diagnosis of Lyme borreliosis can be ascertained by demonstration of borrelial infection using either direct (isolation, PCR) or indirect (serology) microbiological tests. Isolation of B.burgdorferi sensu lato from clinical material represents the most reliable method for confirming borrelial infection. PCR has been developed for the detection of B. burgdorferi sensu lato DNA in clinical specimens. In contrast to culture, PCR is a rapid method but it is not standardized. Serologic tests represent the most commonly used method for establishing microbiological diagnosis of borrelial infection. Specific IgM and IgG antibodies can be detected in blood, CSF and synovial fluid. The percentage of seropositivity increases with duration of infection. It is not possible with serologic tests to distinguish between acute, late, active, or treated disease. In patients with Lyme borreliosis a specific T-cell response to B. burgdorferi sensu lato can also be detectedTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaZdruženje slovenskih dermatovenerologov