{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-5ZRWTVTE/a6952cfc-b206-42be-b782-3a723782e5c6/PDF","dcterms:extent":"101 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-5ZRWTVTE/d4adf86c-1603-4230-9567-b4864f4ca486/TEXT","dcterms:extent":"24 KB"}],"edm:TimeSpan":{"@rdf:about":"1998-2025","edm:begin":{"@xml:lang":"en","#text":"1998"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-5ZRWTVTE","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-KC6O72BG"},{"@xml:lang":"sl","#text":"Acta chimica slovenica"}],"dcterms:issued":"2013","dc:creator":["Barreteau, Hélene","Blanot, Didier","Gobec, Stanislav","Hrast, Martina","Mengin-Lecreulx, Dominique","Sosič, Izidor","Turk, Samo"],"dc:format":[{"@xml:lang":"sl","#text":"številka:2"},{"@xml:lang":"sl","#text":"letnik:60"},{"@xml:lang":"sl","#text":"str. 294-299"}],"dc:identifier":["ISSN:1318-0207","COBISSID:3477105","URN:URN:NBN:SI:doc-5ZRWTVTE"],"dc:language":"en","dc:publisher":[{"@xml:lang":"sl","#text":"Slovenian Chemical Society"},{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"}],"dc:subject":[{"@xml:lang":"en","#text":"Escherichia coli"},{"@xml:lang":"sl","#text":"MurF"},{"@xml:lang":"sl","#text":"peptidoglikani"},{"@xml:lang":"sl","#text":"protibakterijske učinkovine"},{"@xml:lang":"en","#text":"Streptococcus pneumoniae"},{"@xml:lang":"sl","#text":"virtualno rešetanje"},{"@xml:lang":"sl","#text":"zaviralna aktivnost"}],"dcterms:temporal":{"@rdf:resource":"1998-2025"},"dc:title":{"@xml:lang":"sl","#text":"Biochemical characterization of MurF from Streptococcus pneumoniae and the identification of a new MurF inhibitor through ligand-based virtual screening|"},"dc:description":[{"@xml:lang":"sl","#text":"MurF is an essential bacterial enzyme that is involved in the last intracellular stage of peptidoglycan biosynthesis, and therefore it has the potential to be exploited as a target for the development of new antibacterials. Here, we report on the expression, purification and biochemical characterization of MurF from an important pathogen, Streptococcus pneumoniae. Additionally, ligand-based virtual screening was successfully used and a new hit compound with micromolar inhibitory activities against MurF enzymes from S. pneumoniae and Escherichia coli was identified"},{"@xml:lang":"sl","#text":"MurF je esencialen bakterijski encim, ki sodeluje pri zadnji znotrajcelični stopnji biosinteze peptidoglikana in je kot tak obetavna tarča za razvoj novihprotibakterijskih učinkovin. V tem prispevku poročamo o uspešni ekspresiji, izolaciji in biokemijski karakterizaciji encima MurF iz pomembnega patogenega seva Streptococcus pneumoniae. Poleg tega smo tudi uspešno uporabili virtualno rešetanje na osnovi liganda in odkrili novo spojino zadetek z zaviralno aktivnostjo v mikromolarnem območju na encima MurFiz S. pneumoniae in Escherichia coli"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-5ZRWTVTE","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-5ZRWTVTE"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-5ZRWTVTE/a6952cfc-b206-42be-b782-3a723782e5c6/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-5ZRWTVTE/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-5ZRWTVTE"}}}}