4390 KB20 KB199220251998Kuchinka-Koch, A...Schiller, A...Stary, A...številka:3/4letnik:7str. 121-127ISSN:1318-4458COBISSID_HOST:8101337URN:URN:NBN:SI:doc-2YA2NT45enSlovene Welding SocietyActa dermatovenerologica Alpina, Pannonica et AdriaticaCandidaCandidiasisEpidemiologyIsolation and purificationMucous membraneMycosesPrevalenceSaccharomyces cerevisiaeYeastsYeast colonization of mucous membranes: prevalence and identification of different species|The prevalence of different Candida species on mucous membranes of the genital, perigenital, anal, or pharyngeal region was evaluated in 451 male andfemale patients. Furthermore, the reliability of CHROMagar tm Candida for the identification of certain yeast species, such as C. albicans, C. glabrata,C. tropicalis, C. krusei, and Saccharamyces cerevisiae was determined. The most prevalent yeast isolated from the genital tract was C. albicans (86.1%) followed by C. glabrata (8.5%), C. parapsilosis (3.7%), and Saccharomyces cerevisiae (4.2%). The perigenital and anal regions were colonized most frequently with C. albicans (87.5%) only. In contrast to these samples a large variety of different Candida spp. was cultured from the pharyngeal region. Mixed yeast cultures mainly with C. albicans together with C. glabrata or S. cerevisiae were observed more frequently with pharyngeal (11.3%) than with vulvovaginal samples (4.6%). All 408 presumptive C. albicansstrains could be identified on CHROMmagar tm Candida and were confirmed either by rice agar, germ-tube tests, or API resulting in a sensitivity and specificity of 100%, respectively. The same performance characteristics could be observed for C. glabrata, C. krusei, and C. tropicalis. The comparison of different methods demonstrate that CHROMagar tm Candida permits a reliable and higly sensitive as well as specific differentitation of C. albicans, C. glabrata, C. tropicalis, and C. krusei from other Candida spp. by characteristic appearance of colonies within few daysTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaZdruženje slovenskih dermatovenerologov