32 KB484 KB31 KB192920262010Andjelić, SofijaHawlina, MarkoPerovšek, DarkoRobič Pikel, TatjanaZupančič, Gregorletnik:798 stranistr. I-123-I-130številka:suplementISSN:1318-0347COBISSID:27819481URN:URN:NBN:SI:doc-Z11KVV3TslSlovensko zdravniško društvoZdravniški vestnikAcetilholinAcetylcholineCalciumDrug EffectsEpitelijepitelne celiceEpitheliumFura-2Gentian VioletKalcijkataraktalečna ovojnicaLečna ovojnica kristalinskaLens Capsule, CrystallineMetil vijolično barviloMicroscopy, FluorescenceMikroskopija fluorescenčnaoftalmologijaoperativno zdravljenjePhysiologyPhysiopathologyAnterior lens capsule as a tool to study the physiology of human lens epithelial cells| Sprednja lečna ovojnica kot orodje za študij fiziologije lečnih epitelnih celic pri človeku| Sprednja lečna ovojnica kot orodje za študij fiziologije lečnih epitelnih celic pri človeku|Background: Anterior lens capsules excised during routine cataract surgeries are a potential source of the human lens epithelium. The purpose of the work was to check the possibility of using this material to study the physiology and pathophysiology of lens epithelial cells, mainly the homeostasis of the intracellular calcium concentration (šCa2+đi) which serves as an universal indicator of cellular function. Methods: Capsules were isolated during the cataract operations of patients of both genders aged between 35 and 98. Approximately a third of the capsules used were stained during the operation by gentian violet. We monitored the changes in šCa2+đi with the fluorescent dye Fura-2. The standard stimulus applied was acetylcholine. We measured the temporal and spatial changes of the Fura-2 fluorescence in epithelial cells inexperiments done using the system consisting of the inverted fluorescent microscope, the computer controlled light filter changer and the cooled digital CCD camera. Results: Lens epithelial cells typically responded to acetylcholine application with an increase in šCa2+đi expressed as the 360/380ratio of fluorescence. Generally, the responses were strongest in capsules with best morphological appearance. Not all the cells responded simultaneously in acetylcholine responsive capsules. We observed linked responses of local cell networks. Both the capsules that were stained during the surgery with gentian violet and the non-stained ones responded to acetylcholine. In approximately of the capsules epithelial cells contracted in response to acetylcholine application. Conclusions: We found that anterior capsules of the human lens isolated during the cataract surgery are an adequate source for the studies of lens epithelial cells function and can potentially provide important insights into the pathophysiology of the cataractTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko zdravniško društvo