27 KB187 KB19 KB200420252004Rada, VojtěchTrojanová, IvaVlková, Evaštevilka:16 straniletnik:84str. 31-36ISSN:1581-9175COBISSID:1677448ISSN:1854-1941URN:URN:NBN:SI:doc-YY3L09RDenBiotehniška fakultetaActa agriculturae Slovenicabacteriabakterijebifidobakterijeizolacijamicrobiologymikrobiologijamlečni izdelkiEnumeration, isolation, and identification of bifidobacteria from dairy product| Štetje, izolacija in identifikacija bifidobakterij v mlečnih izdelkih|Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l-1). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primersTestirali smo šest mlečnih izdelkov. Štetje bifidobakterij in izolacijo smo izvedli na TPY agarju, ki smo mu dodali muriprocin (100 mg l-1). Izolate smo identificirali na ravni rodu z detekcijo fruktoza-6-fosfat fosfoketolaze (F6PPK) in z metodo FISH. Bifidobakterije smo opisali s hitrima testoma API 50CHL in API ID 32. Preverili smo tudi rast pri 46 °C. Nato smo vse isolate identificirali na ravni vrst z računalniškim programom Bacter. Dodatno smo izvedli identifikacijo z metodo PCR ob uporabi za posamezen rod, oziroma vrsto specifičnih začetnih oligonukleotidovTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Ljubljani, Biotehniška fakulteta