29 KB572 KB26 KB200020242013García, Jorge LópezHumpolíček, PetrJunkar, ItaLehocký, MariánMozetič, Miranštevilka:4letnik:47str. 467-471COBISSID:1004458ISSN:1580-2949URN:URN:NBN:SI:doc-WAJLTBGSenInštitut za kovinske materiale in tehnologijeMateriali in tehnologijeevkariontske celicekeratinocitkolagentanke plastitkivno inženirstvoDifferent source atelocollagen thin films| preparation, process optimisation and its influnece on the interaction with eukaryotic cells| priprava, optimizacija procesa in vpliv na interakcije z evkariontičnimi celicami| Različen izvir atelokolagenskih tankih plasti|Collagen thin films were prepared via bovine atelocollagen matrices. The film casting was carried out by using different culture dishes, concentrations, equipment, drying processes and periods of time. In order to optimise the repeatability and reproducibility, microscopic analyses were utilised to explore the film quality and topographical patterning. In addition, the human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seeded onto the obtained specimens, and the cell proliferation was determined by using the MTT assay. These results indicated how the substrate, its concentration and processing conditions influence the cellular response. The attempted technique shows itself to be an excellent procedure for continuous collagen film preparation with optimal cell-proliferation rates, which may potentially be used in tissue engineering or wound-healing applicationsKolagenska tanka plast je bila narejena z govejo atelokolagensko matriko. Priprava plasti je potekala na različnih gojiščih, pri različnih koncentracijah, opremi, postopkih sušenja in pri različnih časih. Za doseganje optimalne ponovljivosti in obnovljivosti so bile uporabljene mikroskopske analize, s katerimi je bila ugotovljena kakovost plasti in njihov topografski vzorec. Poleg tega so bile na vzorce nanesene imortalizirane nekancerogene celice linije keratinocita (HaCaT), njihova proliferacija pa je bila ugotovljena z MTT-preizkusom. Rezultati prikazujejo, kako podlaga, njena koncentracija ter procesne razmere vplivajo na biološki odziv. Tovrstna tehnika je edinstven postopek za izdelavo kontinuirnih kolagenskih plasti za optimalno proliferacijo celic, ki je potencialno uporabna v tkivnem inženirstvu ali za celjenje ranTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaInštitut za kovinske materiale in tehnologije