31 KB543 KB20 KB200020242002Dahmane, RajaEržen, IdaKreft, MarkoKubínová, LucieSajko, ŠpelaWernig, Antonštevilka:1letnik:216 stranistr. 7-12COBISSID:14502361ISSN:1580-3139URN:URN:NBN:SI:doc-VPRKE6G1enDruštvo za stereologijo in kvantitativno analizo slike, Medicinska fakultetaImage analysis and stereologyAdultAgedAgingAutopsyAvtopsijaCytologyfiziologijaImmunohistochemistryImunohistokemijaMišica skeletnamišiceMuscle, skeletalOdrasliOstareliskeletne mišiceStaranjeImproving methodological strategies for satellite cells counting in human muscle during ageing|Stereological methods, based on the optical disector principle and fluorescentstaining, were developed for estimating frequency of satellite cells in skeletal muscles. The parameter NL(sc, fib) (number of satellite cells per fibre length) was compared with the parameter NN(sc, nucl) (the percentage of satellite cell nuclei in all muscle nuclei), most often published in the literature, by applying unbiased sampling and counting procedures and using a confocal microscope. The methods were tested in autopsysamples of four young vs. four old human vastus lateralis muscles. Bothparameters NL(sc, fb) and NN(sc, nucl) declined during ageing. However, itappears that the two parameters cannot be substituted one by the other because the number of nuclei per fibre length tends to be increased during aging. Using the introduced methods, it is more straightforward to estimate NL(sc, fib) than NN(sc, nucl)TEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo za stereologijo in kvantitativno analizo slike