314 KB47 KB199820252020Bedenk, JureFabjan, TejaKumer, KristinaOsredkar, JoškoVirant-Klun, IrmaVrtačnik-Bokal, Edaštevilka:3letnik:67str. 885-895DOI:10.17344/acsi.2020.5864ISSN:1580-3155COBISSID:24855811URN:URN:NBN:SI:doc-VMM317PGenSlovenian Chemical SocietySlovensko kemijsko društvoActa chimica slovenicaendometriozaoksidativni stresoocitipolicistični jajčnikiAntimüllerian hormone and oxidative stress biomarkers as predictors of successful pregnancy in polycystic ovary syndrome, endometriosis and tubal infertility factor|Oxidative stress in the follicular fluid (FF) is thought to be responsible for the abnormal development of oocytes. In our study patients with polycystic ovarian syndrome (PCOS), endometriosis, and tubal infertility factor (TIF), and healthy women with a male factor of infertility, were prospectively enrolled. From each patient, a sample of individual FF was collected from a dominant follicle. Concentration levels of TAS, 8-IP, 8-OHdG, and AMH were determined. In women with PCOS, we found significantly lower values of oxidative stress markers in the FF. 8-IP and TAS levels were lower in the FF of women with endometriosis. In women with TIF, we also found significantly lower values of all tested markers in the FF, except for 8-OHdG and AMH. We wanted to see whether the biomarker measured in the FF in an individual diagnosis could predict a successfully obtained embryo from this particular follicle. The FF 8-OHdG result in PCOS patients stood out and proved to be a good predictive marker of matured and fertilized oocytes in these patients. Further research is needed to be able to apply the acquired knowledge in improving the outcome of IVF proceduresOksidativni stres v folikularni tekočini (FF) naj bi bil odgovoren za nenormalen razvoj oocitov. V našo raziskavo so bile prospektivno vključene pacientke s sindromom policističnih jajčnikov (PCOS), endometriozo in tubarnim dejavnikom neplodnosti (TIF) ter zdrave ženske z dejavnikom moške neplodnosti. Od vsake bolnice je bil odvzet vzorec FF iz dominantnega folikla. Določene so bile koncentracije TAS, 8-IP, 8-OHdG in AMH. Pri ženskah s PCOS smo ugotovili bistveno nižje vrednosti označevalcev oksidativnega stresa v FF. Stopnje 8-IP in TAS so bile v FF žensk z endometriozo nižje. Pri ženskah s TIF smo ugotovili tudi bistveno nižje vrednosti vseh testiranih označevalcev v FF, razen za 8-OHdG in AMH. Želeli smo videti, ali lahko označevalec, izmerjen v FF pri posamezni diagnozi, napoveduje uspešnost pridobitve zarodka iz tega folikla. Rezultat 8-OHdG v FF pri pacientkah s PCOS je izstopal in se je izkazal za dober napovedni označevalec dozorelih in oplojenih oocitov pri teh pacientkah. Potrebne so nadaljnje raziskave, da bi lahko pridobljeno znanje uporabili za izboljšanje rezultatov postopkov IVFTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko kemijsko društvo