691 KB25 KB200420252023Hajkazemian, MelikaSaidi, Abbasštevilka:1letnik:1198 str.COBISSID_HOST:154871299ISSN:1854-1941URN:URN:NBN:SI:doc-TOMG7LIRenZaložba Univerze v LjubljaniActa agriculturae SlovenicacultivarsDAMDgenetic diversitygenetska raznolikostgenetski markerjiGerberaISSRRAPD markerjiRAPD markerssorteComparative assessment of ISSR, DAMD and RAPD markers for evalu-ation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars|Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD mark-ers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three dis-tinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superior-ity over RAPD and ISSR in characterization of genetic diver-sity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different geno-types of gerbera diversityGenetska raznolikost je izhodišče razpoložljivih genetskih virov v žlahtniteljskih programih gerbere. V študiji smo genetsko raznolikost 22 sort gerbere preučevali z označe-valci ISSR (inter-simple sequence repeat, regije med mikrosa-teliti), DAMD (direct amplified minisatellite DNA, pomnoženi minisateliti) in RAPD (Random Amplified Polymorphic DNA, naključno pomnožena polimorfna DNA). Popreprečna infor-macijska vrednost polimorfizma (PIC) je bila za ISSR, DAMD in RAPD markerje 0,40; 0,41 in 0,40. Klasterska analiza ISSR, DAMD in RAPD markerjev je sorte razdelila v tri različne skupine. Primerjalna analiza treh markerjev (ISSR, DAMD in RAPD) je pokazala, da so imajo DAMD markerji prednost pred RAPD in ISSR za določitev genetske raznolikosti pri ger-beri. Pričujoča raziskava je ena prvih, ki primerja učinkovitost DAMD, ISSR in RAPD markerjev na izbranem naboru geno-tipov gerber. Naši rezultati so pokazali, da so markerji DAMD najbolje prikazali raznolikost genotipov gerbereTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Ljubljani, Biotehniška fakulteta