20 KB156 KB16 KB192920262009Klasinc, MarijaKotnik-Kevorkijan, BoženaLorenčič Robnik, SlavicaPetrovič, ŽivaRupnik, Majaštevilka:3letnik:78str. 119-122ISSN:1318-0347COBISSID:3254335URN:URN:NBN:SI:doc-SC34RWVHenSlovensko zdravniško društvoZdravniški vestnikBacterial Typing TechniquesBakterijska tipizacija, tehnikeBolnišnične okužbeCross InfectionMethicillin Resistantmethicillin-resistant Staphylococcus aureusmethodsMeticilin, odpornostMetodeMRSAPreprečevanje in nadzorprevention and controltipizacijaDiversity of spa types among MRSA isolates from Maribor University hospital| Raznolikost MRSA spa tipov, izoliranih pri bolnikih iz Univerzitetnega kliničnega centra Maribor|Background. Typing of pathogens is an important part in control and preventionof health care-associated infections. For methilcillin-resistant S. aureus (MRSA) a new sequence-based and easily internationally comparable typing method, spa typing, was recently described. We have used this method to analyze the MRSA types present in our hospital and to compare them with types reported elsewhere. Methods. 63 MRSA strains isolated from patients in Maribor University Hospital (MUH) during the year 2006 were spa typed. Typing data were compared to the patient hospitalization data to detect possible spatial and temporal clusters. Results. Sixty-three MRSA strains were distributed into 12 spa types. Seven spa types were represented only by a single isolate. The three most prevalent types (t001, t288, t003) included 80 % of all strains. The most prevalent type, t001, was present in many differentwards and during entire year. This type is also one of the mostly isolated types worldwide. High prevalence of other two types seems to be associated with small scale transmission events. Conclusions. Most of the spa types present in MUH are well known and widespread also elsewhere in Slovenia, in other EU countries and worldwide. Typing has helped us to follow the introduction of different MRSA types to the hospital environment and to detect occasional transmissionsIzhodišča. Tipizacija patogenih mikroorganizmov lahko doprinese k nadzoru in preprečevanju bolnišničnih okužb. Za tipizacijo sevov S. aureus je bila opisana nova metoda, ki temelji na določanju nukleotidnega zaporedja gena spa, ki kodira protein A. Rezultati so zato za razliko od do sedaj uporabljanih metod zelo lahko primerljivi med laboratoriji. Z novo metodo smo zato želeli preiskati seve, izolirane v naši bolnišnici, ugotoviti raznolikost prisotnih tipov ter jih primerjati z drugimi bolnišnicami. Metode. Vsem sevom MRSA, izoliranim pri bolnikih Univerzitetnega kliničnega centra Maribor v letu 2006, smo določili spa tip. Rezultate tipizacije smo nato primerjali s podatki hospitalizacije posameznega bolnika ter tako sledili morebitne časovne in prostorske povezave. Rezultati. Triinšestdeset MRSA sevov smo uvrstili v 12 spa tipov. Sedem spa tipov je imelo le po enega predstavnika. Trije najpogostejši spa tipi (t001, t288, t003) so predstavljali 80 % vseh preiskanih sevov. Najpogostejši tip t001 je bil prisoten na številnih oddelkih v celem letu. Visok delež drugih dveh tipov pa je najverjetneje povezan s prenosi manjšega obsega. Zaključki. Večina MRSA tipov spa, ki so bili prisotni v UKC Maribor v letu 2006, je pogostih tudi drugje v Sloveniji, v Evropi in v svetu. S pomočjo tipizacije smo lahko natančneje sledili vnos MRSA v našo bolnišnico ter prenose znotraj bolnišniceTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko zdravniško društvo