41 KB112 KB25 KB199820252012Bistan, MirjanaMarinšek-Logar, RomanaVodovnik, MašaZorec, Mašaštevilka:1letnik:59str. 83-88ISSN:1318-0207COBISSID:3024008URN:URN:NBN:SI:doc-RPLP8984enSlovenian Chemical SocietySlovensko kemijsko društvoActa chimica slovenicabacteriabakterijecelične membranemicrobiologymikrobiologijaPseudomonas putidatoksične snovitoksikologijaMembrane changes associated with exposure of Pseudomonas putida to selected environmental pollutants and their possible roles in toxicity|A bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess potential effect of five selected chemically diverse environmental pollutants on cell membranes. Long chain fatty acid profiles of cultures exposed to environmentally relevant concentrations of atrazine (ATR), metolachlor (MET), pentachlorobiphenyl (PCB), hexachlorobenzene (HCB) and fluoranthene (FL), were analyzed and compared to non0exposed cultures. To assess sensitivity of membrane-based response, the impact of each toxicant on culture growth was also followed spectrophotometrically. Results revealed changes in fatty acid profiles when cells were exposed to PCB, HCB and FL in concentrations below the inhibitory levels. Moreover, the observed membrane responses were similar to the ones previously associated with adaption to some membrane-active compounds. On the other hand, exposure of cells to any of the two herbicides, ART or MET, did not induce any significant changes in fatty acid profiles. However, when combined with a commonly used fertilizer compound, NHdown4NOdown3, growth impairment was observed. Synergisitc effect of the two herbicides with NHdown4NOdown3, might be a consequence of changes in fatty acid profile increasing membrane fluidity, likely induced by NHdown4+ ionsTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko kemijsko društvo