{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-RH06TF5O/35fb3124-f38a-4e52-a64e-df79ac7706cf/HTML","dcterms:extent":"31 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-RH06TF5O/48e12378-94e7-4f76-9c39-52ade22fd501/PDF","dcterms:extent":"85 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-RH06TF5O/07f83ab5-392e-4553-a096-b26ad056327b/TEXT","dcterms:extent":"23 KB"}],"edm:TimeSpan":{"@rdf:about":"2000-2024","edm:begin":{"@xml:lang":"en","#text":"2000"},"edm:end":{"@xml:lang":"en","#text":"2024"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-RH06TF5O","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-fzwbu7gu"},{"@xml:lang":"sl","#text":"Image analysis and stereology"}],"dcterms:issued":"2002","dc:creator":["Marcos, Ricardo","Monteiro, Rogério A. F.","Rocha, Eduardo"],"dc:format":[{"@xml:lang":"sl","#text":"številka:1"},{"@xml:lang":"sl","#text":"letnik:21"},{"@xml:lang":"sl","#text":"6 strani"},{"@xml:lang":"sl","#text":"str. 1-6"}],"dc:identifier":["COBISSID:14502105","ISSN:1580-3139","URN:URN:NBN:SI:doc-RH06TF5O"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Društvo za stereologijo in kvantitativno analizo slike, Medicinska fakulteta"},"dc:subject":[{"@xml:lang":"sl","#text":"citologija"},{"@xml:lang":"sl","#text":"imunohistokemija"},{"@xml:lang":"sl","#text":"jetra"},{"@xml:lang":"sl","#text":"podgane"},{"@xml:lang":"sl","#text":"stereologija"},{"@rdf:resource":"http://www.wikidata.org/entity/Q9368"}],"dcterms:temporal":{"@rdf:resource":"2000-2024"},"dc:title":{"@xml:lang":"sl","#text":"Stereological estimation of Ito cells from rat liver using the optical fractionator - a preliminary report|"},"dc:description":{"@xml:lang":"sl","#text":"In the last two decades, much light has been shed on hepatic fibrosis, and theactivation/proliferation of Ito cells (IC) emerged to play a central role. Therefore, it is essential to have solid quantitative data in nonpathological statuses; yet, this data is scarce and conned to \"number per area\" or semiquantitative information. Moreover, the supposed heterogeneous distribution of IC in the hepatic lobule was never analysed with design-based (unbiased) stereology. In the present study, the total number (N) of IC in ratliver was estimated for the %rst time, by combining immunocytochemistry with the optical fractionator. Quantification was extended to the hepatocytes,to disclose the IC index, an often-used ratio in hepatology. Systematic uniform random liver sections were obtained from male Wistar rats (n = 3), and immunostained against glial %brillary acidic protein (GFAP), a known specific marker for hepatic IC. For the first time, these were marked against GFAP in thick (30 pm) paraffin sections. The estimated N of IC was 224E06; with a coefficient of error of 0.04 or 0.06, depending on the particular equation used (based on the so-called \"quadratic approximation\"). The IC index was 91 IC/1000 hepatocytes. Concerning the lobular heterogeneity,it was proved the liver harbours a larger total number of periportal IC and hepatocytes"},"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-RH06TF5O","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-RH06TF5O"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-RH06TF5O/48e12378-94e7-4f76-9c39-52ade22fd501/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Društvo za stereologijo in kvantitativno analizo slike"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-RH06TF5O/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-RH06TF5O"}}}}