28 KB279 KB26 KB192920262002Čižek-Sajko, MojcaVlaisavljević, Veljkoletnik:71str. I-25-I-29številka:supl. 1ISSN:1318-0347COBISSID:856639URN:URN:NBN:SI:doc-QELELJGXslSlovensko zdravniško društvoZdravniški vestnikCelična kulturaCell cultureEmbryoFertilization in vitrofiziologijahumana reprodukcijain vitro fertilizationjajčna celicaJajčnik, folikelOploditev in vitroOvarian follicleZarodekzunajtelesna oploditevZnačilnosti razmnoževanja kumulusnih celic dominantnega folikla v in vitro pogojih| The proliferation of dominant follicle cumules cells in in vitro conditions|We studied the proliferative capacity of dominant follicle culumus cells under in vitro conditions in natural in vitro fertilization (IVF) cycles. We attempted to determine whether the bahavior of cumulus cells in culture medium is also reflected in the fertilization capacity of the oocyte and embryo quality. Methods. In 69 patients undergoing IVF, the oocyte - cumulus complex was obtained by follicle puncture. On the day following the puncture, cumulus cell morphology and fertilization were evaluated. After three days of culture, the proliferation of cumulus cells was evaluated and on the day of embryo transfer, i. e. on the third day after puncture, embryo quality also. Results. After one day of culture, the transformation of cumulus cells from spherical to stellate form was noted in 87.0% cell samples, in 5.8% of samples the cells adhered to the bottom but retained their spherical form and in 7.2% of samples the cumulus cells remained in the suspension. After three days of culture, the cumulus cells exhibited good proliferation in 65.2% of samples, in 34.8% of samples proliferation was minimal or absent. The comparison of cumulus cell transformation after one day of culture and proliferation evaluated after three days of culture revealed their correlation. No relationship was found between the proliferative capacity of cumulus cells and fertilization, but we were able to confirm the relationship between the proliferative capacity of cumulus cells and embryo morphology. Conclusions. Under in vitro conditions, cumulus cells express a different capacity of morphological transformation and proliferation. From the pattern of cumulus cells proliferation we can infer to the embryo quality but not to the fertilization capacity of the oocyteIzhodišča. Proučevali smo sposobnost razmnoževanja kumulusnih celic dominantnega folikla v in vitro pogojih pri naravnih ciklih zunajtelesne oploditve. Zanimalo nas je, ali se obnašanje kumulusnih celic v kulturi odslikava tudi v oploditveni sposobnosti jajčne celice in v kakovosti zarodka. Metode. Pri 69 preiskovankah smo v postopku zunajtelesne oploditve s punkcijo folikla dobili kompleks jajčna celica - kumulus. Dan po punkciji smo ocenili obliko kumulusnih celic in oploditev. Po treh dneh kultiviranja smo ocenili razmnoževanje kumulusnih celic in na dan prenosa zarodka, to je tretji dan po punkciji, ovrednotili tudi kakovost zarodka. Rezultati. Spreminjuanje oblike kumulusnih celic iz kroglaste v zvezdasto smo po enem dnevu kultiviranja opazili pri 87,0% vzorcev celic, pri 5,8% vzorcev so se celice pritrdile na podlago, vendar so ohranile kroglasto obliko, pri 7,2% vzorcev pa so kumulusne celice ostale v suspenziji. Po treh dneh kultiviranja so se kumulusne celice dobro razmnoževale pri 65,2% vzorcev, pri 34,8% vzorcev pa je bilo razmnoževanje le neznatno oziroma ga ni bilo. Primerjava spreminjanja oblike kumulusnih celic po enem dnevu kultiviranja in razmnoževanja, ocenjenega po treh dneh kultiviranja, je pokazala njuno medsebojno povezanost. Nismo našli povezanosti med sposobnostjo razmnoževanja kumulusnih celic na oploditveno sposobnost jajčne celice, lahko pa smo potrdili odvisnost med sposobnostjo razmnoževanja kumulusnih celic in morfologijo zarodka. Zaključki. Kumulusne celice v in vitro pogojih izražajo različno sposobnost spreminjanja oblike in različno sposobnost razmnoževanja. Na podlagi vzorca razmnoževanja kumulusnih celic lahko sklepamo na kakovost zarodka, ne pa tudi na oploditveno sposobnost jajčne celiceTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko zdravniško društvo