{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-PHLQSJFZ/71281f90-625e-4707-bb11-f47bc9506a37/PDF","dcterms:extent":"1995 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-PHLQSJFZ/66decc03-9216-4d6d-92a2-e7ff3e1ba48e/TEXT","dcterms:extent":"57 KB"}],"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-PHLQSJFZ","dcterms:issued":"2026","dc:creator":["Čemažar, Maja","Kamenšek, Urška","Kupčič, Saša","Lampreht Tratar, Urša"],"dc:format":[{"@xml:lang":"sl","#text":"številka:3"},{"@xml:lang":"sl","#text":"letnik:60"},{"@xml:lang":"sl","#text":"str. 402-415"}],"dc:identifier":["DOI:10.2478/raon-2026-0049","ISSN:1318-2099","COBISSID_HOST:290269699","URN:URN:NBN:SI:doc-PHLQSJFZ"],"dc:language":"en","dc:publisher":[{"@xml:lang":"sl","#text":"Croatian Medical Association - Croatian Society of Radiology"},{"@xml:lang":"sl","#text":"Slovenian Medical Society - Section of Radiology"}],"dc:source":{"@xml:lang":"sl","#text":"Radiology and oncology (Ljubljana)"},"dc:subject":[{"@xml:lang":"en","#text":"bioluminescence"},{"@xml:lang":"sl","#text":"bioluminiscenca"},{"@xml:lang":"en","#text":"luciferase"},{"@xml:lang":"sl","#text":"luciferaza"},{"@xml:lang":"sl","#text":"mišji ortotopni tumor"},{"@xml:lang":"en","#text":"mouse orthotopic tumor"},{"@xml:lang":"en","#text":"osteosarcoma"},{"@xml:lang":"sl","#text":"osteosarkom"}],"dc:title":{"@xml:lang":"sl","#text":"Luciferase-induced immune activation prevents orthotopic K7M2luc osteosarcoma establishment in BALB/c mice|"},"dc:description":{"@xml:lang":"sl","#text":"Background. Use of murine orthotopic tumor models dictates utilization of luciferase-expressing cells for in vivo biolu-minescence imaging. However, luciferase may trigger immune responses that impair tumor establishment.Materials and methods. We transduced K7M2 cells wild-type (wt) with luciferase gene and evaluated their growth in vitro. We then implanted cells orthotopically in BALB/c mice and upon unsuccessful tumor establishment assessed systemical and local mechanism for tumor growth suppression.Results. All investigated cell lines had a comparative doubling time in vitro at 24 and 48h. Despite that, K7M2luc E5 differed from K7M2luc A7 and K7M2 wt by having a much higher proliferation rate at 72h (35x, 15x and 17x). In vivo, both luciferase-expressing cell lines failed to establish tumors, whereas K7M2 wt had a 100% success rate. To investi-gate the underlying mechanism, we assessed systemic and local cell-mediated immunity. Mice in groups K7M2luc A7 and K7M2luc E5 had a higher fold change (5 ± 0.8 and 6 ± 1.05) of GrB secreting splenocytes than naive mice (1 ± 0.2). In the bone microenvironment, luciferase groups exhibited elevated numbers of GrB-secreting cells and CD8+T cells relative to K7M2 wt and naive mice.Conclusions. These findings indicate that luciferase activates cytotoxic T-cell responses capable of eliminating im-planted cells. Tumor establishment was restored in immunodeficient mice lacking CD8+ cells, confirming the immune-mediated rejection"},"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-PHLQSJFZ","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-PHLQSJFZ"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-PHLQSJFZ/71281f90-625e-4707-bb11-f47bc9506a37/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Društvo radiologije in onkologije"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-PHLQSJFZ/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-PHLQSJFZ"}}}}