491 KB22 KB199320242004Boškova, TatjanaĆurčić, Božidar P. M.Makarov, Slobodan E.Nikolić, GoranRomac, Stankaštevilka:1letnik:12str. 89-98ISSN:1318-1998COBISSID:387061URN:URN:NBN:SI:doc-O4WODXDZenPrirodoslovni muzej SlovenijeSlovensko entomološko društvo Štefana MichielijaActa entomologica slovenica (Ljubljana)biokemijadoločanjeelektroforeza proteinovinsektiElectrophoretic patterns of body extract proteins in some diplopods (Diplopoda, Myriapoda)|The technique of the universal film agarose electrophoresis has been used to detect biochemical characteristics of the body extract proteins in 7 different species and 7 genera of the diplopod families Julidae, Craspedosomatidae, and Polydesmidae. The migration rate of these proteins as well as their relative abundance for Julus terrestris Linnaeus (Julidae), Melogona broelemanni Verhoeff (Craspedosomatidae), Pachyiulus hungaricus Karsch, Unciger foetidus (C. L. Koch), Leptoiulus trilineatus (C. L. Koch), Megaphyllum unilineatum (C. L. Koch) (Julidae), and Brachydesmus dadayii Verhoeff (Polydesmidae) were compared. Electrophoretic identifications of these species showed both species-specific and (probably) intergeneric differences. It is assumed that body protein electrophoresis can be also used: (a) to differentiate species in any stage of their life cycle, (b) to reveal the presence of sibling species, and to differentiate the taxonomic and evolutionary interrelations both in congeneric as well as in other, more distant taxaZa ugotavljanje biokemičnih značilnosti proteinov telesnih izvlečkov 7 različnih vrst in 7 rodov dvojnonog družin Julidae, Craspedosomatidae in Polydesmidae smo uporabili tehniko splošne tankoplastne gelske elektroforeze. Primerjali smo stopnje selitev proteinov in njihovo relativno pogostnost pri vrstah Julus terrestris Linnaeus (Julidae), Melogona broelemanni Verhoeff (Craspedosomatidae), Pachyiulus hungaricus Karsch, Unciger foetidus (C. L. Koch), Leptoiulus trilineatus (C. L. Koch), Megaphyllum unilineatum (C. L. Koch) (Julidae) in Brachydesmus dadayii Verhoeff (Polydesmidae). Elektroforezne določitve teh vrst so pokazale vrstno specifične in (verjetno) medrodovne razlike. Predvidevamo, da je elektroforeza telesnih proteinov uporabna za (a) razločevanje vrst v katerem koli razvojnem stadiju, (b) razkrivanju prisotnosti sestrskih vrst in ugotavljanju taksonomskih in evolucijskih odnosov v okviru rodov, kot tudi med bolj oddaljenimi taksoniTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaPrirodoslovni muzej Slovenije