17 KB141 KB14 KB199220252004Geraj-Vrhovac, VeraZeljezić, Davorštevilka:1letnik:385 stranistr. 43-47ISSN:1318-2099COBISSID:17663449URN:URN:NBN:SI:doc-NYK9R988enAssociation of Radiology and OncologyRadiology and oncology (Ljubljana)AdultCobalt RadioisotopesDNADna DamageDNA, pošdkobaDose-Response Relationship, RadiationDoza-odgovor, odnos, sevanjeGama žarkiGamma RaysgenomKobaltovi radioizotopiLimfocitiLymphocytesobsevanjeOdrasliRadiation EffectsComet assay in the assessment of the human genome damage induced by gamma-radiation in vitro|Background. The aim of the present study was to estimate a possible application of comet assay in the evaluation of DNA damage caused by differentgamma radiation doses in peripheral human lymphocytes in vitro. Materials and methods. Whole blood samples of young healthy, non-smoking donors were taken. The samples were divided in 4 specimens. The first specimenwas used as the control. Other three specimens were irradiated using constant gamma irradiation source (60Co) giving the dose rate of 0.907 cGy/s. Different specimens were irradiated for 51 s, 437 s and 1099 s, giving the doses of 0.5 Gy, 4 Gy and 10 Gy. In order to estimate dose-response curve on the control and all 3 irradiated whole blood samples, the comet assay under alkali conditions was performed. Results and conclusions. The comet assay endpoints showed statistically significantly higher values for all irradiated blood samples compared to the control. For both, tail length and tail moment, dose-effect relationship was found to be linear in a dose range of 0.5Gy and 10 Gy. By this work we also pointed out possible usage of the comet assay in the detection of DNA lesions caused by extremely high radiarion dose, which isnot possible by using standard cytogenetic methodsNamen. Z raziskavo smo želeli preveriti možnost uporabe testa komet pri ocenjevanju poškodb DNK, ki jih povzročijo različne doze žarkov x na perifernih človeških limfocitih in vitro. Materiali in metode. Čiste krvne vzorce smo odvzeli mladim, zdravim prostovoljcem - nekadilcem. Vzorce smo razdelili v 4 skupine. Prvo smo uporabili kot kontrolo, ostale tri pa smo obsevali z napravo s konstantnim obsevalnim virom žarkov gama (60Co) s hitrostjo doze 0,907 cGy/s. Skupine krvnih vzorcev smo obsevali 51 sekund, 437sekund in 1099 sekund z ustrezajočimi dozami 0,5 Gy, 4 Gy in 10 Gy. Za oceno krivulj, ki so ponazarjale odgovor kontrolne skupine vzorcev in treh obsevanih skupin vzorcev na obsevanje, smo opravili kometni test v alkalnih pogojih. Rezultati in zaključki. Izid kometnega testa je pokazal statistično pomembno višje vrednosti pri obsevanih skupinah vzorcev v primerjavi s kontrolno skupino. Učinek doze v razponu od 0,5 Gy do 10 Gy je bil premosorazmeren tako z dolžino kometovega repa kot z momentom. Z raziskavo smotudi potrdili, da je kometni test mogoče uporabiti za odkrivanje poškodb DNK, ki jih povzročijo visoke obsevalne doze. Takšnih poškodb s citogenetskimimetodami ni mogoče odkritiTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo radiologije in onkologije