1448 KB2026Ahmed, Alaa NaserAlasadi, Ramiz Mahdi SalihAlnaqeb, HaithamTuaimah, Mohammed Hadištevilka:110 str.letnik:122DOI:10.14720/aas.2026.122.1.22362ISSN:1854-1941COBISSID_HOST:271090691URN:URN:NBN:SI:doc-NXV4U199enZaložba Univerze v LjubljaniActa agriculturae Slovenicacalluscontaminationdate palm treedatljeva palmafungiglivekaluskontaminacijapoganjkishootstissue culturetkivna kulturaInfluence of pH of culture medium on fungal contaminants and growth response of Barhi cultivar date palm explants (Phoenix dactylifera L.) in vitro|Date palm is commonly propagated through tissue culture techniques; however, this method often encounters complications, particularly fungal contamination. The effects of varying pH levels on fungal development and explant response in vitro are the focus of the research. Morphological and molecular assessments led to the identification of three fungal species: Alternaria alternata (Fr.) Keissl., Chaetomium globosum Kunze and Nigrospora osmanthi Mei Wang & L. Cai were the most prevalent, with A. alternata being the most frequently observed. Among the tested pH levels, pH 5 had the most pronounced impact on the radial expansion of the fungi. In terms of shoot development within the tissue culture environment, overall shoot growth of date palm was not significantly influenced by pH levels. However, shoot length exhibited notable differences at pH 5, 5.5, and 6, measuring 4.2 cm, 4.6 cm, and 5.1 cm, respectively. Furthermore, the amino acid concentration in leaf tissues varied with pH, peaking at 50.6 mg per 100 g of tissue at pH 5. Carbohydrate levels in the leaves were also pH-dependent, with the highest value of 29.26 mg g-1 at pH 6 and the lowest (0.34 mg g-1) at pH 5TEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Ljubljani, Biotehniška fakulteta