{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-NCU2WXSO/7e97aa03-fd53-4cd4-a497-f51c73487285/HTML","dcterms:extent":"18 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-NCU2WXSO/45cfe8b6-e4ea-4c82-b664-68939f8b87ce/PDF","dcterms:extent":"140 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-NCU2WXSO/d090f27d-359d-4157-9b59-213fecc2e2d7/TEXT","dcterms:extent":"17 KB"}],"edm:TimeSpan":{"@rdf:about":"2002-2026","edm:begin":{"@xml:lang":"en","#text":"2002"},"edm:end":{"@xml:lang":"en","#text":"2026"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-NCU2WXSO","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-py0w57lr"},{"@xml:lang":"sl","#text":"Zdravstveno varstvo"}],"dcterms:issued":"2004","dc:creator":["Kotnik, Antonija","Prosenc, Katarina","Sočan, Maja","Šubelj, Vesna"],"dc:format":[{"@xml:lang":"sl","#text":"številka:2"},{"@xml:lang":"sl","#text":"letnik:43"},{"@xml:lang":"sl","#text":"str. 99-103"}],"dc:identifier":["ISSN:0351-0026","COBISSID:1209829","URN:URN:NBN:SI:doc-NCU2WXSO"],"dc:language":"sl","dc:publisher":{"@xml:lang":"sl","#text":"Inštitut za varovanje zdravja Republike Slovenije"},"dc:subject":[{"@xml:lang":"en","#text":"diagnostika"},{"@xml:lang":"sl","#text":"ELISA"},{"@xml:lang":"sl","#text":"influenca"}],"dcterms:temporal":{"@rdf:resource":"2002-2026"},"dc:title":{"@xml:lang":"sl","#text":"Primerjava dveh presejalnih metod za odkrivanje influence A, influnce B in RSV| Comparison of ELISA and multiplex RT-PCR screening assays for detection of influenza A, influenza B and RSV| ELISA in multipleks RT-PCR|"},"dc:description":[{"@xml:lang":"sl","#text":"Acute respiratory tract infections (ARI) are the leading cause of increased morbidity and mortality in winter months. Because of nonspecific clinical symptoms of influenza, laboratory confirmation is essential for surveying influenza spread in the community. Rapid tests, such as enzyme linked immunoassay (ELISA) and multiplex reverse transcription-polymerase chain reaction (RT-PCR), were introduced to replace the time-consuming isolation of influenza and RSV viruses. Multiplex RT-PCR allows for detection of three different antigens during a single test. The test was adapted to enable reverse transcription and DNA replication to be processed in the same reactionmix. Throat and nasal swabs were obtained from out-patients and in-patients with ARI. ELISA identified influenza A in 2.5%, influenza B in 6.3% and RSV in 2% of the 605 clinical samples tested. Detection rates achieved by RT-PCR were 3.6%, 18.6 % and 3%, respectively. For isolation in cell culture all ELISA and/or RT-PCR-positive samples were used. Influenza A virus was recovered from two, influenza B from 33 and RSV from five samples. RT-PCR was found to be a more sensitive test than ELISA. It improved the rateof virus isolation from RT-PCR-positive samples, which is of utmost importance for typification and sub-typification of influenza viruses. Higher sensitivity of RT-PCR was established in all age groups. RT-PCR yielded betterdetection results than ELISA, especially in the age group 5 years and over. Despite its higher cost and greater expertise required, RT-PCR offers a significant advantage over ELISA, and is superior to it in influenza screeningand RSV detection"},{"@xml:lang":"sl","#text":"V zimskem času so akutne okužbe dihal vzrok izrazitega porasta obolevnosti in umrljivosti. Med pomembnejšimi povzročitelji so virusi influence in RSV. Zaradi neznačilne klinične slike je potrebno kroženje teh virusov potrditi z virološkimi metodami. Ker je osamitev virusov v celični kulturi zahtevna in dolgotrajna, skušamo prve podatke dobiti s hitrejšimi metodami. Kot presejalnimetodi sta bili obravnavni encimsko-imunski test (ELISA) in multipleksna verižna reakcija s polimerazo z reverznim prepisom (RT-PCR). Slednja omogoča odkrivanje več antigenov hkrati. Prilagojena je bila tako, da sta reverzni prepis in pomnoževanje DNK potekala s pripravo ene reakcijske mešanice. Brise žrela in nosu so pri bolnikih s klinično sliko gripi podobne bolezni odvzeli zdravniki, ki sodelujejo v mreži za epidemiološko in virološkospremljanje gripe in zdravniki Klinike za infekcijske bolezni in vročinska stanja v Ljubljani. Obdelanih je bilo 605 vzorcev. Z ELISA je bil dokazan virus influence A v 2,5%, virus influence B v 6,3% in RSV v 2% vzorcev, z RT-PCR pa genom influence A v 3,6%, influence B v 18,6% in RSV v 3%vzorcev. Iz vzorcev, ki so bili pozitivni s katero koli od uporabljenih tehnik, je bilo namnoževanje virusov v celični kulturi za virus influence A uspešno iz 2 vzorcev, za virus influence B iz 33 in za RSV iz 5 vzorcev. Ugotovljeno je bilo, da je presejalna metoda RT-PCR bolj občutljiva kot ELISA in da poveča tudi delež uspešnih osamitev v celičnih kulturah, kar je izrednega pomena za tipizacijo in subtipizacijo virusov influence. Tehnika RT-PCR je bolj občutljiva od tehnike ELISA v vseh starostnih skupinah, razlikapa se v višjih starostnih skupinah povečuje v prid RT-PCR. Posebej je opazno povečanje možnosti odkrivanja virusov influence in RSV z RT-PCR v starostnih skupinah nad 5 let. Kljub večji zahtevnosti in višji ceni se je RT-PCR izkazal za primernejšo presejalno metodo kot ELISA"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-NCU2WXSO","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-NCU2WXSO"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-NCU2WXSO/45cfe8b6-e4ea-4c82-b664-68939f8b87ce/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Inštitut za varovanje zdravja RS"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-NCU2WXSO/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-NCU2WXSO"}}}}