68 KB1648 KB37 KB200020242006Balatsouka, DimitraGotfredsen, KlausGundersen, Hans Jorgen G.številka:112 straniletnik:25str. 13-24ISSN:1580-3139COBISSID:21169369URN:URN:NBN:SI:doc-NA75TB65enDruštvo za stereologijo in kvantitativno analizo slike, Medicinska fakultetaImage analysis and stereologyAnatomy And HistologyBone DensityBone ScrewshistologijaImage Processing, Computer-AssistedimplantatiKost, gostotakostikostna gostotaKostni vijakiOseointegracijaOsseointegrationRabbitsSlika, obdelava z računalnikomstereologijaTibiaTibijaTitanTitaniumZajciEvaluation of bone-to-implant contact and bone density adjacent to titanium implants using a stereological technique on ground sections|When bone implants have to be examined in situ ground sections are required. Histomorphometric measurements are usually performed on two-dimensional sections, causing biased results when they are wrongly extrapolated to 3D without any knowledge of stereology. Unbiased results can only be obtained using stereological principles. The aim of the study was to describe an unbiased design for evaluating bone-to-implant contact (BIC) and peri-implant bone density (BD-i) in three-dimensions. The unbiased design was based on a fixed axis vertical random sampling technique. Three bone-implant blocks were collected from 3 rabbits. Four sections were obtained from each animal using afixed axis vertical random sampling technique. The BIC was estimated by creating a stereological method based on a systematic test line set. The BD-i was estimated using a design based on a systematic point set. The efficiency of the systematic sampling was evaluated for each sampling level: Coefficient of error CE(wi) for the systematic test line set, CESyst between the 4 rotatedsections, CENoise for the reproducibility. These variances were compared to the biological variation (CV) between animals. The mean CE(Wi) was5 to 6%; the CESyst was 7.8% for the BIC estimates and 5.7% for the BD-i estimates; the CENoise was 5.8% for the BIC and 7.7% for the BD-i. The CV was 19% for the BIC estimates and 24% for the BD-i estimates. These results demonstrated that the stereological technique used in the present study was a very efficient method to obtain unbiased estimates of BIC and BD-i in 3D on 2Dimplant-bone sectionsTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo za stereologijo in kvantitativno analizo slike