130 KB41 KB199420252004Rajtar, Simonaštevilka:4letnik:43str. 339-349ISSN:0025-8121COBISSID:19160793URN:URN:NBN:SI:doc-MRZGP2IVslMedicinski razglediMedicinski razglediAmitriptilinAmitriptylineBloodCatsChromatography, High Pressure LiquidChromatography, LiquidDrug EffectsfarmakokinetikaHistaminHistamin, sproščanjeHistamineHistamine ReleasekriKromatografija tekočinskaKromatografija visokotlačna, tekočinskaMačkeSpectrometry, FluorescenceSpektrometrija fluorescenčnaVpliv amitriptilina in sertralina na kinetiko eksogenega histamina v krvi mačke v in vitro poskusih| The influence of amitriptyline and sertraline in vitro on the kinetics of exogenous histamine in cat blood|Histamine is a biogenic amine known as a primary mediator of allergic reactions. In brain, its role involves neurotransmission. Researches in the field of histamine pharmacokinetics have shown that antidepressants modify thepharmacokinetics of exogenous histamine in cats and rats. They decrease elevated histamine concentrations in plasma after an injection of exogenous histamine. Consequently, animals which have not been treated with antidepressants have significantly elevated histamine concentrations after an intravenous injection of this amine. In order to determine the extent to whichblood cells participate in the modification of exogenous histamine kinetics, in vitro tests were performed on cat blood. After taking blood samples, the plasma was separated from blood cells and exogenous histamine andtwo antidepressants, amitriptyline and sertraline, were added to the blood cells in vitro. The concentration of histamine in the samples was determined spectroflourometrically, while methylhistamine was determined using HPLC. The results indicate that both amitriptyline and sertraline in vitro increase the reuptake of exogenous histamine in cat blood cells, although the effect of sertraline is more significant than that of amitriptylineHistamin je biogeni amin in je znan kot primarni mediator pri alergijskih reakcijah. V možganih ima funkcijo živčnega prenašalca. Raziskave na področju farmakokinetike histamina so pokazale, da antidepresivi spremenijo farmakokinetiko eksogenega histamina pri mački in podgani, in sicer zmanjšajo porast koncentracije histamina v plazmi po vbrizganju eksogenega histamina, zaradi katerega se pri živalih, ki niso tretirane z antidepresivi, pojavi močno zvišana koncentracija histamina po intravenskem vbrizganju tega amina. Da bi ugotovili, v kolikšni meri pri spremembi kinetike eksogenega histamina zaradi delujočega antidepresiva sodelujejo krvne celice, smo naredili in vitroposkuse na mačji krvi. Po odvzemu krvi iz mačke smo ločili plazmo od krvnih celic in celicam in vitro dodajali eksogeni histamin in antidepresiva amitriptilinin in sertralin. Koncentracijo histamina v vzorcih smo določali sprektrofluorometrično, metilhistamina pa s HPI,C. Rezultati so pokazali, da tako amitriptilin kot sertralin in vitro povečata privzem eksogenega histaminav krvne celice mačke. Učinek sertralina na privzem histamina je večjiod učinka amitriptilinaTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo Medicinski razgledi