46 KB247 KB27 KB200420252004Dovč, PeterHabe, FrancKavar, Tatjanaštevilka:2letnik:849 stranistr. 131-139ISSN:1581-9175COBISSID:1676680ISSN:1854-1941URN:URN:NBN:SI:doc-LSC4CNL2slBiotehniška fakultetaActa agriculturae SlovenicaDNAkonjilipicancimolekularna genetikapasmerodovnikiRodovi lipicancev slovenske reje glede na haplotip mitohondrijske DNK| Mitochondrial DNA haplotypes in Slovenian Lippizzan mare families|Sequencing of mitochondrial DNA from 53 Slovenian Lipizzans revealed 17 distinct mtDNA haplotzpes: ten haplotypes (Capriola, Allegra, Monteaura, Slavina, Batosta, Gratiosa, Wera, Betalka, Dubovina and Gaetana) were present in classical mare families, haplotype Thais in the new Slovenian mare family Rebecca and haplotypes C, M, Strana, Trompeta, Boka and Y in mare families of Croatian, Romanian or Hungarian origin. We expected large amount of "classical" haplotypes, because after 1947 the Lipizzan breed in Slovenia was re-established using mainly Lipizzans from classical mare families. However, Lipizzans from incorrect mare families were probably used for the recovery of three classical families and therefore in these three families Slovenian Lipizzans had different haplotypes than other European Lipizzans. Additional pedigree errors were detected in four families showing more than one haplotype. We suggested that pedigree errors should be corrected by introduction of several new mare families and by minor re-distributions of Lipizzans among mare families. Geographical origin of the Lipizzan haplotypes remains uncertain, but haplotypes Allegra and Monteaura could be of Spanish origin due to the highest frequency of these haplotypes in Iberian (Andalusian and Lusitano) and North African horses (Barbs). Kladrubian origin of haplotypes Batosta and Slavina, which are characteristic for Lipizzan mare families of Kladrubian origin, Africa and Almerina, could be supported by the presence of both haplotypes in the present Kladruber horses. The Arabic origin of haplotype Gaetana, which is characteristic for the Lipizzan mare family of Arabic origin, could be supported by the high frequency of the haplotype Gaetana in Arabian horsesZ analizo mitohondrijske DNK 53 lipicancev slovenske reje smo našli 17 različnih haplotipov mitohondrijske DNK. Deset haplotipov (Capriola, Allegra, Monteaura, Slavina, Batosta, Gratiosa, Wera, Betalka, Dubovina in Gaetana) je bilo značilnih za klasične lipicanske rodove, haplotip Thais za nov slovenski rod Rebecca in haplotipi M, C, Strana, Trompeta, Boka in Y za lipicanske rodove hrvaškega, romunskega ali madžarskega izvora. Visok delež haplotipov, značilnih za klasične rodove, je bil pričakovan, ker so v Sloveniji po letu 1947 želeli obnoviti lipicansko čredo predvsem s klasičnimi rodovi. Rezultati kažejo, da so bili trije klasični rodovi obnovljeni z lipicanci iz napačnih rodov; v teh rodovih imajo lipicanci slovenske reje namreč različne haplotipe kot lipicanci iz drugih evropskih držav. Več kot en haplotip smo našli znotraj štirih rodov, kar kaže na dodatne nepravilnosti v rodovnikih. Predlagamo, da se nepravilnosti odpravijo z uvedbo novih rodov in z manjšimi prerazporeditvami lipicancev med rodovi. Geografski izvor lipicanskih haplotipov ostaja večinoma neznan, toda haplotipa Allegra in Monteaura bi bila lahko španskega izvora, ker so taki haplotipi najbolj pogosti pri iberijskih (andaluzijskih in lusitano konjih) ter severno-afriških konjih (berberih). Kladrubski izvor haplotipov Batosta in Slavina, ki sta značilna za lipicanska rodova kladrubskega izvora Africa in Almerina, lahko potrdimo z odkritjem obeh haplotipov pri današnjih kladrubskih konjih; arabski izvor haplotipa Gaetana, ki je značilen ya lipicanski rod arabskega izvora Gidrane, pa z visoko pogostostjo haplotipa Gaetana v populaciji arabskih konjTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Ljubljani, Biotehniška fakulteta