53880 KB189 KB0 KB2022Knez Marevci, MašaKotnik, PetraŽitek, TajaVIII, 172 str., 30 cmCOBISSID:136547587URN:URN:NBN:SI:doc-HMUJ0CX0slT. Žitekvisokošolska delaanticancer potentialantikancerogeni potencialantimicrobial potentialantimikrobni potencialDisertacijeekstrahiranjeextractionformulationformuliranjeIngverNavadna konopljaoptimizationoptimiziranjeseedingsejanjeZdravilnostOptimizacija procesa izolacije učinkovin iz industrijske konoplje v kombinaciji z ingverjem ter biološki učinki mešanic| doktorska disertacija|The doctoral dissertation describes optimized pretreatment and processing from raw material to final product. It lists the pretreatment procedures of industrial hemp to achieve a higher purity of the material even before extraction. It also focuses on optimizing the extraction method and the possibility of a synergistic effect between compounds from industrial hemp and plants of the Zingiberaceae family. The quality of the extract and the synergy between the plants is characterized based on analytical results (determination of cannabinoids), with the computer program Design expert, the computer language R, and the determination of antioxidant, antimicrobial and anticancer activity. Finally, the possibility of a natural formulation that meets the criteria of shelf life and preserves the bioavailability of the formulated extract is presented.In the doctoral dissertation, it was confirmed the significant effect of mechanical pretreatment of the raw material on the content of cannabinoids, resulting in a significantly higher purity of the extract. It was found that by combining supercritical extraction with carbon dioxide (temperature 60 °C, pressure between 300 bar and 550 bar) and ethanolic extraction with ultrasound (temperature 8 °C, time 8 minutes), significantly approached the cannabinoid content in the plant while obtaining high levels of cannabinoids, especially the CBD component (511 mg/g extract). Ginger also proved to have a significant effect on industrial hemp, as a synergistic effect occurred upon extraction of their mixture, increasing the content of the CBD component by 11.5%. In addition, it was also confirmed the antimicrobial potential for the bacteria Staphylococcus aureus and Escherichia coli, as well as the fungus Candida albicans. We also demonstrated the inhibition of WM -266-4 melanoma cells when the mixture extract or the cannabis extract was applied. However, regarding antimicrobial and anticancer potential, the mixture extract showed more effectiveness and inhibition of microorganisms and cancer cells, further confirmed by an apoptosis assay. Finally, it was prepared a natural formulation of the extract in the form of an organogel. The main advantage of organogel systems is low toxicity and irritability after application to human skin, and more active penetration of active substances to the target site. It was confirmed the optimal composition: 18% extract, 5% gelator (Stearic acid) and 77% cold-pressed hemp oil.In the dissertation, we present procedures for producing a high-quality natural product with high selectivity and positive effects on the human body in a "green" process. Based on the results, we assume that the obtained extract or organogel is suitable for supportive therapy in skin diseasesDoktorska disertacija opisuje optimiziran postopek predelave in obdelave naravnega materiala od surovega materiala do končnega produkta. Navaja postopke predpriprave industrijske konoplje za dosego višje selektivnosti materiala že pred ekstrakcijo. Nato se osredotoča na optimizacijo ekstrakcijske metode in možnosti sinergističnega učinka med komponentami industrijske konoplje in komponentami iz rastlin družine Zingiberaceae. Kvaliteto ekstrakta in sinergijo med rastlinami karakterizira na podlagi analitskih rezultatov (determinacija kanabinoidov), z računalniškim programom Design expert, računalniškim jezikom R ter determinacijo antioksidativnega, antimikrobnega in antikancerogenega delovanja. Na koncu pa predstavi možnost naravne formulacije, ki ustreza kriterijem obstojnosti in ohrani biološko razpoložljivost formuliranega ekstrakta.V doktorski disertaciji je bil potrjen signifikanten vpliv postopka mehanske predobdelave surovega materiala na vsebnost kanabinoidov, zaradi katerega je posledično bila čistost ekstrakta bistveno višja. Ugotovljeno je bilo, da se s kombinacijo ekstrakcije s superkritičnim ogljikovim dioksidom (pri temperaturi 60 °C in tlaku med 300 bar in 550 bar) ter ultrazvočno etanolno ekstrakcijo, izvedeno pri temperaturi 8 °C in 8 min, pomembno približamo kanabinoidnemu razmerju v rastlini, hkrati pa dosežemo visoke vsebnosti kanabinoidov, predvsem CBD komponente (511 mg/g ekstrakta). Prav tako se je izkazalo, da ima ingver pomemben sinergističen vpliv na industrijsko konopljo, saj se je vsebnost CBD komponente v ekstraktu povišala za 11,5 ut. %. Dobljenemu ekstraktu je bil potrjen antimikrobni potencial za bakteriji Staphylococcus aureus in Escherichia coli ter glivo Candida albicans. Dokazana je bila tudi inhibicija melanomskih celic WM-266-4 ob nanosu ekstrakta mešanice ali ekstrakta konoplje. Glede na antimikrobni in antikancerogeni potencial je ekstrakt mešanice pokazal aktivnejšo delovanje oziroma zaviranje mikroorganizmov in rakastih celic, kar smo dodatno potrdili z apoptotskim testom. Na koncu smo pripravili naravno formulacijo ekstrakta v obliki organogela. Glavna prednost organogelskih sistemov sta nizka toksičnost in iritabilnost po aplikaciji na človeško kožo ter aktivnejšo penetracijo učinkovin na tarčno mesto. Kot optimalno sestavo smo potrdili: 18 % ekstrakta, 5 % gelatorja (Stearinske kisline) in 77 % hladno stiskanega konopljinega olja.Doktorska disertacija skozi 'zelen' proces predstavlja postopke za pripravo kakovostnega naravnega produkta visoke selektivnosti s pozitivnimi vplivi na človeško telo. Glede na rezultate zaključuje, da je pridobljen ekstrakt ali organogel primeren za uporabo kot podporna terapija pri različnih boleznihTEXTvisokošolska delatheses and dissertationsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Mariboru, Fakulteta za kemijo in kemijsko tehnologijo