27 KB326 KB18 KB200020242000Brown, Sheena MMercerl, Alison RNapper, Ruth Mletnik:19številka:26 stranistr. 139-144COBISSID:11708121ISSN:1580-3139URN:URN:NBN:SI:doc-H7CDO2ZUenDruštvo za stereologijo in kvantitativno analizo slike, Medicinska fakultetaImage analysis and stereologyAgingBeesCentral nervous systemcentralni živčni sistemčebeleelektronska mikroskopijaHistological techniqueshistologijametodeMicroscopy, electron, scanning transmissionMicroscopy, polarizationmožganiNeuronal plasticitySynapsesAnalysis of structural plasticity in the honey bee brain using the Cavalieri estimator of volume and the disector method|The antennal lobe of the worker honey bee has been used as a model system to address the origins of structural plasticity in the central nervous system. A combination of light and electron microscopy was used to determine total synapse number within an easily identifiable sub-unit of the antennal lobe neuropil, the T4-2(1) glomerulus. The Cavalieri method was applied at the light microscope level to determine a reference volume (Vref) of this glomerulus. Using transmission electron microscopy, the physical disector was used to determine synaptic density (Nv) within the T4-2(1) glomerulus. An estimate of the total synapse number N(syn) was determined by; N(syn) = V(ref)Nv. Newly emerged, 4-day old,10-day old and forager-aged bees were analysed. Results showed that despite a significant increase in T4-2(1) volumewith age, the total number of synapses in this glomerulus did not show acorresponding increase. Disturbingly, it is possible that huge variances within age groups, due to one or two outlying data points, could be masking the true trend of synapse number. This variance, the heterogeneous distribution of synapses within this glomerulus and the problems associated with reproducibility of synapse counts are discussedTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo za stereologijo in kvantitativno analizo slike