{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-F0M0QKT2/cb874e6d-017f-4a40-a8f6-c525ee5d23cd/HTML","dcterms:extent":"48 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-F0M0QKT2/ac8d7803-1591-4de0-88ee-7354bd3fde9b/PDF","dcterms:extent":"280 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-F0M0QKT2/7de567a9-9af0-4ba3-90cb-284cc205ed72/TEXT","dcterms:extent":"37 KB"}],"edm:TimeSpan":{"@rdf:about":"1998-2025","edm:begin":{"@xml:lang":"en","#text":"1998"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-F0M0QKT2","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-KC6O72BG"},{"@xml:lang":"sl","#text":"Acta chimica slovenica"}],"dcterms:issued":"2010","dc:creator":["Brodnjak-Vončina, Darinka","Cencič-Kodba, Zdenka","Novič, Marjana","Potočnik, Uroš"],"dc:format":[{"@xml:lang":"sl","#text":"številka:3"},{"@xml:lang":"sl","#text":"letnik:57"},{"@xml:lang":"sl","#text":"str. 571-580"}],"dc:identifier":["ISSN:1318-0207","COBISSID:14417942","URN:URN:NBN:SI:doc-F0M0QKT2"],"dc:language":"en","dc:publisher":[{"@xml:lang":"sl","#text":"Slovenian Chemical Society"},{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"}],"dc:subject":[{"@xml:lang":"sl","#text":"bolezni"},{"@xml:lang":"sl","#text":"celične membrane"},{"@xml:lang":"en","#text":"chemistry"},{"@xml:lang":"en","#text":"disease"},{"@xml:lang":"sl","#text":"fosfolipidi"},{"@xml:lang":"sl","#text":"kemija"},{"@xml:lang":"sl","#text":"maščobne kisline"},{"@xml:lang":"sl","#text":"večvariantna analiza"}],"dcterms:temporal":{"@rdf:resource":"1998-2025"},"dc:title":{"@xml:lang":"sl","#text":"Multivariate data analysis of erythrocyte membrane phospholipid fatty acid profiles in the discrimination between normal blood tissue and various disease states|"},"dc:description":[{"@xml:lang":"sl","#text":"The investigation presented here aims to compare the fatty acid composition of red blood cells of patients with different disease states and to test the hypothesis that the changes in fatty acid profiles derived from erythrocyte phospholipids might be relevant to various diseases. The study sample consisted of 342 blood donors, among them 135 with inflammatory bowel disease, 53 with uterine leiomyoma, 14 with verified absence of uterine leiomyoma, 52 with asthma, 18 with colon adenomas, and 70 blood samples without any of mentioned diseases that was used as a control group. After the isolation of erythrocytes from blood samples, total extracted lipids were separated by solid-phase extraction (SPE) into non polar lipids and polar phospholipids. After the saponification of phospholipid fraction, the esterification process followed with boron trifluoride-methanol reagent. The fatty acid methyl ester (FAME) composition of the total red blood cell phospholipid fraction was analyzed by gas chromatography (GC) with flame ionization detector (FID). Additionally two fatty aldehyde dimethyl acetals (hexadecanal and octadecanal dimethyl acetals; 16:0 DMA and 18:0 DMA) derived from erythrocyte membrane plasmalogen phospholipids were also determined. The resulting fatty acid and plasmalogen linked fatty acid composition was evaluated by the principal component analysis (PCA). We demonstrated decreased levels of omega-3 polyunsaturated fatty acids (n-3 PUFAs) in red blood cell membrane of patients with colon adenomas. Also, a large negative correlation was observed among all samples between the quantity of saturated acids and arachidonic (20:4n6) acid as well as saturated acids and adrenic (22:4n6) acid. In PCA score plot a group of female donors is distinguished mainly by the content of linoleic (18:2n6) acid; a small subgroup shows its concentration highly above the average value. At the same time, the same subgroup has both dimethyl acetals below the average concentrations. The study demonstrates feasibility of multivariate data analysis in discrimination of patients with different diseases according to fatty acid profile and suggests considerable differences in membrane fatty acid profiles in patients with various disease states"},{"@xml:lang":"sl","#text":"V prispevku je predstavljena študija primerjave sestave maščobnih kislin iz fosfolipidov človeških membran rdečih krvnih celic bolnikov z izbranimi bolezenskimi stanji z namenom testiranja hipoteze, da je sprememba v sestavi lahko povezana z različnimi boleznimi. V študijo je bilo vključenih 342 posameznikov, od tega 135 bolnikov s kronično vnetno črevesno boleznijo, 53 bolnic z miomi maternice in 14 žensk dokazano brez miornov maternice, nadalje 52 bolnikov z astmo, 18 bolnikov s črevesnimi polipi in 70 zdravih posameznikov (krvodajalcev), ki so služili kot kontrolna skupina. Izolaciji rdečih krvnih celic je sledilo ločevanje celokupnih ekstrahiranih lipidov z ekstrakcijo na trdni fazi (SPE) na nepolarne in polarne lipide. Po umiljenju fosfolipidne frakcije je sledila esterifikacija z bor trifluorid metanolnim reagentom. Sestavo metil estrov mašcobnih kislin skupne fosfolipidne frakcije rdečih krvnih celic smo določali s plinsko kromatografijo s plamensko ionizacijskim detektorjem. Dodatno smo iz membran rdečih krvnih celic fosfolipidnih plazmalogenov določili tudi dva dimetil acetala (heksadekanal in oktadekanal dimetil acetal; 16:0DMA in 18:0DMA). Z metodo glavnih osi smo karakterizirali vzorce glede na sestavo mašcobnih kislin in dimetil acetalov, povezanih s plazmalogeni. Ugotovili smo zelo nizke vsebnosti omega-3 polinenasičenih maššobnih kislin v celičnih membranah rdečih krvnih celic pri pacientih s črevesnimi polipi. Velik negativni korelacijski koeficient je bil ugotovljen med vsebnostjo nasičenih maščobnih kislin in arahidonsko (20:4n6) kislino in med vsebnostjo nasičenih mašcobnih kislin in adrensko (22:4n6) kislino. Metoda glavnih osi je pokazala manjšo izstopajočo skupino pacientov ženskega spola z različnim bolezenskim fenotipom, ki se je ločila glede na vsebnost linolne (18:2n6) kisline, ki je bila pri tej podskupini precej nad povprecno vrednostjo vseh 342 vzorcev. Študija je pokazala, da obstajajo razlike v sestavi maščobnih kislin rdečih krvnih celic pri pacientih z različnimi boleznimi"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-F0M0QKT2","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-F0M0QKT2"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-F0M0QKT2/ac8d7803-1591-4de0-88ee-7354bd3fde9b/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-F0M0QKT2/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-F0M0QKT2"}}}}